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Targeting of prolamins by RNAi in bread wheat: Effectiveness of seven silencing-fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins

Opened Access Targeting of prolamins by RNAi in bread wheat: Effectiveness of seven silencing-fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins

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Autor: Barro, Francisco
Iehisa, Julio C. M.
Giménez, María J.
García Molina, María D.
Ozuna, Carmen V.
Comino Montilla, Isabel María
Sousa Martín, Carolina
Gil Humanes, Javier
Departamento: Universidad de Sevilla. Departamento de Microbiología y Parasitología
Fecha: 2016
Publicado en: Plant Biotechnology Journal, 14 (3), 986-996.
Tipo de documento: Artículo
Resumen: Gluten proteins are responsible for the viscoelastic properties of wheat flour but also for triggering pathologies in susceptible individuals, of which coeliac disease (CD) and noncoeliac gluten sensitivity may affect up to 8% of the population. The only effective treatment for affected persons is a strict gluten-free diet. Here, we report the effectiveness of seven plasmid combinations, encompassing RNAi fragments from a-, c-, x-gliadins, and LMW glutenin subunits, for silencing the expression of different prolamin fractions. Silencing patterns of transgenic lines were analysed by gel electrophoresis, RP-HPLC and mass spectrometry (LC-MS/ MS), whereas gluten immunogenicity was assayed by an anti-gliadin 33-mer monoclonal antibody (moAb). Plasmid combinations 1 and 2 downregulated only c- and a-gliadins, respectively. Four plasmid combinations were highly effective in the silencing of x-gliadins and c-gliadins, and three of these also silenced a-gliadins. HMW glutenins were upregulate...
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Cita: Barro, F., Iehisa, J.C.M., Giménez, M.J., García Molina, M.D., Ozuna, C.V., Comino Montilla, I.M.,...,Gil Humanes, J. (2016). Targeting of prolamins by RNAi in bread wheat: Effectiveness of seven silencing-fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins. Plant Biotechnology Journal, 14 (3), 986-996.
Tamaño: 261.9Kb
Formato: PDF

URI: http://hdl.handle.net/11441/41888

DOI: http://dx.doi.org/10.1111/pbi.12455

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