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Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network

Opened Access Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network
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Autor: Muñiz Guinea, Manuel
Martín Rubio, María Esther
Hidalgo Jiménez, Josefina
Velasco López, Ángel
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Fecha: 1997
Publicado en: Proceedings of the National Academy of Sciences of the USA, 94 (26), 14461–14466
Tipo de documento: Artículo
Resumen: We have examined the role played by protein kinase A (PKA) in vesicle-mediated protein transport from the trans-Golgi network (TGN) to the cell surface. In vivo this transport step was inhibited by inhibitors of PKA catalytic subunits (C-PKA) such as the compound known as H89 and a myristoylated form of the inhibitory peptide sequence contained in the thermostable PKA inhibitor. Inhibition by H89 occurred at an early stage during the transfer of vesicular stomatitis virus G glycoprotein from the TGN to the cell surface. Reversal from this inhibition correlated with a transient increase in the number of free coated vesicles in the Golgi area. Vesicle budding from the TGN was studied in vitro using vesicular stomatitis virus-infected, permeabilized cells. Addition to this assay of C-PKA stimulated vesicle release while it was suppressed by PKA inhibitory peptide, H89, and antibody against C-PKA. Furthermore, vesicle release was decreased when PKA-depleted cytosol was used and restored b...
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Tamaño: 392.8Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40526

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