Artículo
Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network
Autor/es | Muñiz Guinea, Manuel
Martín Rubio, María Esther Hidalgo Jiménez, Josefina Velasco López, Ángel |
Director | |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 1997 |
Fecha de depósito | 2016-04-27 |
Publicado en |
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Resumen | We have examined the role played by protein kinase A (PKA) in vesicle-mediated protein transport from the trans-Golgi network (TGN) to the cell surface. In vivo this transport step was inhibited by inhibitors of PKA catalytic ... We have examined the role played by protein kinase A (PKA) in vesicle-mediated protein transport from the trans-Golgi network (TGN) to the cell surface. In vivo this transport step was inhibited by inhibitors of PKA catalytic subunits (C-PKA) such as the compound known as H89 and a myristoylated form of the inhibitory peptide sequence contained in the thermostable PKA inhibitor. Inhibition by H89 occurred at an early stage during the transfer of vesicular stomatitis virus G glycoprotein from the TGN to the cell surface. Reversal from this inhibition correlated with a transient increase in the number of free coated vesicles in the Golgi area. Vesicle budding from the TGN was studied in vitro using vesicular stomatitis virus-infected, permeabilized cells. Addition to this assay of C-PKA stimulated vesicle release while it was suppressed by PKA inhibitory peptide, H89, and antibody against C-PKA. Furthermore, vesicle release was decreased when PKA-depleted cytosol was used and restored by addition of C-PKA. These results indicate a regulatory role for PKA activity in the production of constitutive transport vesicles from the TGN. |
Cita | Muñiz Guinea, M., Martín Rubio, M.E., Hidalgo Jiménez, J. y Velasco López, Á. (1997). Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network. Procedings of the National Academy of Sciences of the United States of America, 94 (26), 14461-14466. |
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