Opened Access Carotene-superproducing strains of Phycomyces
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Autor: Murillo Araujo, Francisco José
López Calderón, Isabel
López Díaz, Isabel
Cerdá Olmedo, Enrique
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 1978
Publicado en: Applied and Environmental Microbiology, 36 (5), 639-642
Tipo de documento: Artículo
Resumen: Production of fl-carotene by wild-type Phycomyces blakesleeanus can be stimulated by light, chemicals, regulatory mutations, and sexual interaction between mycelia of opposite sex. Through genetic manipulations, we have isolated strains which have simultaneously and constitutively incorporated several of these stimulatory effects. In the dark and in a simple medium, some of the strains produce up to 25 mg of f-carotene per g (dry weight), or about 500 times the wild-type production under the same conditions. High lycopene-producing strains have also been isolated by using carR mutants, which are blocked in the conversion of lycopene to fl-carotene. These strains should be useful in both industrial production of these pigments and basic research related to carotenogenesis.
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