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Saccharomyces cerevisiae Aqr1 is an internal-membrane transporter involved in excretion of amino acids

Opened Access Saccharomyces cerevisiae Aqr1 is an internal-membrane transporter involved in excretion of amino acids

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Autor: Velasco, Isabel
Tenreiro, Sandra
López Calderón, Isabel
André, Brunó
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2004
Publicado en: Eukaryotic Cell, 3 (6), 1492-1503
Tipo de documento: Artículo
Resumen: Excretion of amino acids by yeast cells was reported long ago but has not been characterized in molecular terms. It is typically favored by overproduction of the amino acid and/or impairment of its uptake. Here we describe the construction of a yeast strain excreting threonine and homoserine. Using this excretor strain, we then applied a reverse-genetics approach and found that the transporter encoded by the YNL065w/AQR1 gene, a protein thought to mediate H antiport, is involved in homoserine and threonine excretion. Furthermore, overexpression of AQR1 led to increased excretion of several amino acids (alanine, aspartate, and glutamate) known to be relatively abundant in the cytosol. Transcription of the AQR1 gene is induced severalfold by a number of amino acids and appears to be under the negative control of Gcn4. An Aqr1-green fluorescent protein fusion protein is located in multiple internal membrane structures and appears to cycle continuously between these com...
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Tamaño: 350.6Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40236

DOI: http://dx.doi.org/10.1128/EC.3.6.1492–1503.2004

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