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Intergeneric hybrids of Saccharomyces cerevisiae and Zygosaccharomyces fermentati obtained by protoplast fusion

Opened Access Intergeneric hybrids of Saccharomyces cerevisiae and Zygosaccharomyces fermentati obtained by protoplast fusion
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Autor: Pina, A.
López Calderón, Isabel
Benítez, Tahía
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 1986
Publicado en: Applied and Environmental Microbiology, 51 (5), 995-1003
Tipo de documento: Artículo
Resumen: To obtain strains that are able to efficiently produce ethanol from different carbohydrates, mainly cellulose hydrolysates, several species of the genus Candida and a Zygosaccharomyces fermentati strain were examined for their ability to utilize cellobiose and produce ethanol, as well as for their thermotolerance and the possibility of genetic manipulation. Candida obtusa and Zygosaccharomycesfermentati tolerated the maximal temperature for growth, possessed the highest cellobiase activity, and offered the possibility of genetic manipulation, although neither of them proved to be a good producer of ethanol. Intergeneric hybrids of Saccharomyces cerevisiae and Z. fermentati were obtained after protoplast fusion. They were selected as prototrophic strains, after isolation of auxotrophic mutants from Z. fermentati and fusion with an S. cerevisiae strain which was also ...
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Tamaño: 1.737Mb
Formato: PDF

URI: http://hdl.handle.net/11441/40235

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