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Artículo
Threonine overproduction in yeast strains carrying the HOM3-R2 mutant allele under the control of different inducible promoters
dc.creator | Farfán Espuny, María José | es |
dc.creator | Aparicio, Luis | es |
dc.creator | López Calderón, Isabel | es |
dc.date.accessioned | 2016-04-21T10:29:20Z | |
dc.date.available | 2016-04-21T10:29:20Z | |
dc.date.issued | 1999 | |
dc.identifier.issn | 1098-5336 | es |
dc.identifier.uri | http://hdl.handle.net/11441/40234 | |
dc.description.abstract | The HOM3 gene of Saccharomyces cerevisiae codes for aspartate kinase, which plays a crucial role in the regulation of the metabolic flux that leads to threonine biosynthesis. With the aim of obtaining yeast strains able to overproduce threonine in a controlled way, we have placed the HOM3-R2 mutant allele, which causes expression of a feedback-insensitive enzyme, under the control of four distinctive regulatable yeast promoters, namely, P GAL1 ,P CHA1 ,P CYC1-HSE2 , and P GPH1 . The amino acid contents of strains bearing the different con- structs were analyzed both under repression and induction conditions. Although some differences in overall threonine production were found, a maximum of around 400 nmol/mg (dry weight) was observed. Other factors, such as excretion to the medium and activity of the catabolic threonine/serine deaminase, also affect threonine accumulation. Thus, improvement of threonine productivity by yeast cells would probably require manipula- tion of these and other factors. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Applied and Environmental Microbiology, 65 (1), 110-116 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Threonine overproduction in yeast strains carrying the HOM3-R2 mutant allele under the control of different inducible promoters | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://aem.asm.org/content/65/1/110.full.pdf+html | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40234 |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Threonine overproduction.pdf | 104.2Kb | [PDF] | Ver/ | |