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Threonine overproduction in yeast strains carrying the HOM3-R2 mutant allele under the control of different inducible promoters

Opened Access Threonine overproduction in yeast strains carrying the HOM3-R2 mutant allele under the control of different inducible promoters
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Autor: Farfán Espuny, María José
Aparicio, Luis
López Calderón, Isabel
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 1999
Publicado en: Applied and Environmental Microbiology, 65 (1), 110-116
Tipo de documento: Artículo
Resumen: The HOM3 gene of Saccharomyces cerevisiae codes for aspartate kinase, which plays a crucial role in the regulation of the metabolic flux that leads to threonine biosynthesis. With the aim of obtaining yeast strains able to overproduce threonine in a controlled way, we have placed the HOM3-R2 mutant allele, which causes expression of a feedback-insensitive enzyme, under the control of four distinctive regulatable yeast promoters, namely, P GAL1 ,P CHA1 ,P CYC1-HSE2 , and P GPH1 . The amino acid contents of strains bearing the different con- structs were analyzed both under repression and induction conditions. Although some differences in overall threonine production were found, a maximum of around 400 nmol/mg (dry weight) was observed. Other factors, such as excretion to the medium and activity of the catabolic threonine/serine deaminase, also affect threonine accumulation. Thus, improvement of threonine productivity by yeast cells would probably requi...
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Tamaño: 104.2Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40234

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