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Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from uncaria tomentosa bark on human ewing's sarcoma and breast cancer cell lines
dc.creator | García Giménez, María Dolores | |
dc.creator | García Prado, Elena | |
dc.creator | Sáenz Rodríguez, María Teresa | |
dc.creator | Fernández Arche, María de los Ángeles | |
dc.creator | Puerta Vázquez, Rocío de la | |
dc.date.accessioned | 2016-03-22T09:10:51Z | |
dc.date.available | 2016-03-22T09:10:51Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0032-0943 | es |
dc.identifier.uri | http://hdl.handle.net/11441/38905 | |
dc.description.abstract | Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peru- vian traditional medicine for the treatment of in- fective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mi- traphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphyl- line was differentially identified from its stereoisomeric pair isomitraphylline by 15N‑NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewingʼs sarcoma MHH‑ES‑1 and breast cancer MT-3 cell lines, using cyclo- phosphamide and vincristine as reference con- trols. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)- 2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic assay. Micromolar concentrations of mitraphylline (5 µM to 40 µM) inhibited the growth of both cell lines in a dose- dependent manner. The IC50 ± SE values were 17.15 ± 0.82 µM for MHH‑ES‑1 and 11.80 ± 1.03 µM for MT-3 for 30 hours, smaller than those obtained for the reference compounds. This ac- tion suggests that the pentacyclic oxindole alka- loid mitraphylline might be a new promising agent in the treatment of both human sarcoma and breast cancer. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Georg Thieme Verlag | es |
dc.relation.ispartof | Planta Medica, 76, 133-136 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Uncaria tomentosa | es |
dc.subject | Rubiaceae | es |
dc.subject | Catʼs claw | es |
dc.subject | Oxindole alkaloid | es |
dc.subject | Mitraphylline | es |
dc.subject | Cytotoxic activity | es |
dc.title | Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from uncaria tomentosa bark on human ewing's sarcoma and breast cancer cell lines | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Farmacología | es |
dc.relation.publisherversion | 10.1055/s-0029-1186048 | es |
dc.identifier.doi | http://dx.doi.org/10.1055/s-0029-1186048 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/38905 |
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