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Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from uncaria tomentosa bark on human ewing's sarcoma and breast cancer cell lines

Opened Access Cytotoxic effect of the pentacyclic oxindole alkaloid mitraphylline isolated from uncaria tomentosa bark on human ewing's sarcoma and breast cancer cell lines

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Autor: García Giménez, María Dolores
García Prado, Elena
Sáenz Rodríguez, María Teresa
Fernández Arche, María de los Ángeles
Puerta Vázquez-Zafra, Rocío de la
Departamento: Universidad de Sevilla. Departamento de Farmacología
Fecha: 2010
Publicado en: Planta Medica, 76, 133-136
Tipo de documento: Artículo
Resumen: Preparations from Uncaria tomentosa, a South American Rubiaceae, have been used in the Peru- vian traditional medicine for the treatment of in- fective, inflammatory and tumoral processes. In this study, the pentacyclic oxindole alkaloid mi- traphylline was isolated from the dried inner bark of this plant species, and its structure elucidated by analysis of NMR spectroscopic data. Mitraphyl- line was differentially identified from its stereoisomeric pair isomitraphylline by 15N‑NMR. Its antiproliferative and cytotoxic effects have been tested on human Ewingʼs sarcoma MHH‑ES‑1 and breast cancer MT-3 cell lines, using cyclo- phosphamide and vincristine as reference con- trols. A Coulter counter was used to determine viable cell numbers, followed by the application of the tetrazolium compound MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy phenyl)- 2-(4-sulfophenyl)-2H-tetrazolium] an inner salt. A colorimetric method was employed to evaluate cell viability in this cytotoxic ...
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Tamaño: 92.31Kb
Formato: PDF

URI: http://hdl.handle.net/11441/38905

DOI: http://dx.doi.org/10.1055/s-0029-1186048

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