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Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein

Opened Access Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein

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Autor: Ramos Morales, Francisco
Infante, Carlos R.
Fedriani Iriso, Concepción
Bornens, Michel
Ríos, Rosa M.
Departamento: Universidad de Sevilla. Departamento de Genética
Universidad de Sevilla. Departamento de Microbiología
Fecha: 1999
Publicado en: Journal of Cell Biology, 145 (1), 83-98
Tipo de documento: Artículo
Resumen: We report that a peripheral Golgi protein with a molecular mass of 210 kD localized at the cis- Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C. Antony, M.C. Boissier, J.C. Homberg, and M. Bornens. 1994. J. Cell Biol. 125:997Ð1013) is a microtubule-binding protein that associates in situ with a subpopulation of stable microtubules. Interaction of this protein, now called GMAP-210, for Golgi microtubule-associated protein 210, with microtubules in vitro is direct, tight and nucleotide-independent. Biochemical analysis further suggests that GMAP-210 specifically binds to microtubule ends. The full-length cDNA encoding GMAP-210 predicts a protein of 1,979 amino acids with a very long central coiled-coil domain. Deletion analyses in vitro show that the COOH terminus of GMAP- 210 binds to microtubules whereas the NH 2 terminus binds to Golgi membranes. Overexpression of GMAP- 210Ðencoding cDNA induced a dramatic enlargement of the Golgi apparatus and perturbation...
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Tamaño: 680.9Kb
Formato: PDF

URI: http://hdl.handle.net/11441/36688

DOI: 10.1083/jcb.145.1.83

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