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Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi

Opened Access Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi

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Autor: Avalos Cordero, Francisco Javier
Limón Mirón, María del Carmen
Díaz Sánchez, Violeta
Departamento: Universidad de Sevilla. Departamento de Genética
Fecha: 2012
Publicado en: Applied and Environmental Microbiology, 78, 7258-7266
Tipo de documento: Artículo
Resumen: Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen avail- ability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduc- tion of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wil...
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Tamaño: 2.128Mb
Formato: PDF

URI: http://hdl.handle.net/11441/36412

DOI: http://dx.doi.org/10.1128/AEM.01552-12

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