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Artículo
The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination
dc.creator | Ávalos Cordero, Francisco Javier | |
dc.creator | García Martínez, Jorge | |
dc.creator | Brunk, Michael | |
dc.creator | Terpitz, Ulrich | |
dc.date.accessioned | 2016-02-23T08:25:40Z | |
dc.date.available | 2016-02-23T08:25:40Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 2045-2322 | es |
dc.identifier.uri | http://hdl.handle.net/11441/36312 | |
dc.description.abstract | Rhodopsins are membrane-embedded photoreceptors found in all major taxonomic kingdoms using retinal as their chromophore. They play well-known functions in different biological systems, but their roles in fungi remain unknown. The filamentous fungus Fusarium fujikuroi contains two putative rhodopsins, CarO and OpsA. The gene carO is light-regulated, and the predicted polypeptide contains all conserved residues required for proton pumping. We aimed to elucidate the expression and cellular location of the fungal rhodopsin CarO, its presumed proton-pumping activity and the possible effect of such function on F. fujikuroi growth. In electrophysiology experiments we confirmed that CarO is a green-light driven proton pump. Visualization of fluorescent CarO-YFP expressed in F. fujikuroi under control of its native promoter revealed higher accumulation in spores (conidia) produced by light-exposed mycelia. Germination analyses of conidia from carO2 mutant and carO1 control strains showed a faster development of light-exposed carO2 germlings. In conclusion, CarO is an active proton pump, abundant in light-formed conidia, whose activity slows down early hyphal development under light. Interestingly, CarO-related rhodopsins are typically found in plant-associated fungi, where green light dominates the phyllosphere. Our data provide the first reliable clue on a possible biological role of a fungal rhodopsin | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Nature Publishing Group | es |
dc.relation.ispartof | Scientific Reports, 5, 7798 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | 10.1038/srep07798 | es |
dc.identifier.doi | http://dx.doi.org/10.1038/srep07798 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/36312 |
Ficheros | Tamaño | Formato | Ver | Descripción |
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THE CARO RHODOPSIN.pdf | 1.226Mb | [PDF] | Ver/ | |