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The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination

 

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dc.creator Ávalos Cordero, Francisco Javier
dc.creator García Martínez, Jorge
dc.creator Brunk, Michael
dc.creator Terpitz, Ulrich
dc.date.accessioned 2016-02-23T08:25:40Z
dc.date.available 2016-02-23T08:25:40Z
dc.date.issued 2015
dc.identifier.issn 2045-2322 es
dc.identifier.uri http://hdl.handle.net/11441/36312
dc.description.abstract Rhodopsins are membrane-embedded photoreceptors found in all major taxonomic kingdoms using retinal as their chromophore. They play well-known functions in different biological systems, but their roles in fungi remain unknown. The filamentous fungus Fusarium fujikuroi contains two putative rhodopsins, CarO and OpsA. The gene carO is light-regulated, and the predicted polypeptide contains all conserved residues required for proton pumping. We aimed to elucidate the expression and cellular location of the fungal rhodopsin CarO, its presumed proton-pumping activity and the possible effect of such function on F. fujikuroi growth. In electrophysiology experiments we confirmed that CarO is a green-light driven proton pump. Visualization of fluorescent CarO-YFP expressed in F. fujikuroi under control of its native promoter revealed higher accumulation in spores (conidia) produced by light-exposed mycelia. Germination analyses of conidia from carO2 mutant and carO1 control strains showed a faster development of light-exposed carO2 germlings. In conclusion, CarO is an active proton pump, abundant in light-formed conidia, whose activity slows down early hyphal development under light. Interestingly, CarO-related rhodopsins are typically found in plant-associated fungi, where green light dominates the phyllosphere. Our data provide the first reliable clue on a possible biological role of a fungal rhodopsin es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Nature Publishing Group es
dc.relation.ispartof Scientific Reports, 5,  7798 es
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title The CarO rhodopsin of the fungus Fusarium fujikuroi is a light-driven proton pump that retards spore germination es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
dc.relation.publisherversion 10.1038/srep07798 es
dc.identifier.doi http://dx.doi.org/10.1038/srep07798 es
dc.identifier.idus https://idus.us.es/xmlui/handle/11441/36312
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