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Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics

Opened Access Differential regulation of soluble and membrane-bound inorganic pyrophosphatases in the photosynthetic bacterium Rhodospirillum rubrum provides insights into pyrophosphate-based stress bioenergetics
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Autor: Losada Villasante, Manuel
López Marqués, Rosa Laura
Pérez Castiñeira, José Román
Serrano Delgado, Aurelio
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2004
Publicado en: Journal of Bacteriology, 186(16), 5418–5426
Tipo de documento: Artículo
Resumen: Soluble and membrane-bound inorganic pyrophosphatases (sPPase and H -PPase, respectively) of the purple nonsulfur bacterium Rhodospirillum rubrum are differentially regulated by environmental growth conditions. Both proteins and their transcripts were found in cells of anaerobic phototrophic batch cultures along all growth phases, although they displayed different time patterns. However, in aerobic cells that grow in the dark, which exhibited the highest growth rates, Northern and Western blot analyses as well as activity assays demonstrated high sPPase levels but no H -PPase. It is noteworthy that H -PPase is highly expressed in aerobic cells under acute salt stress (1 M NaCl). H -PPase was also present in anaerobic cells growing at reduced rates in the dark under either fermentative or anaerobic respiratory conditions. Since H -PPase was detected not only under all anaerobic growth conditions but also under salt stress in aerobiosis, the corresponding gene is not invariably...
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Tamaño: 1.125Mb
Formato: PDF

URI: http://hdl.handle.net/11441/26062

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