dc.creator | Araujo Garrido, Juan Luis | es |
dc.creator | Baisón Olmo, Fernando | es |
dc.creator | Bernal Bayard, Joaquín | es |
dc.creator | Romero Portillo, Francisco | es |
dc.creator | Ramos Morales, Francisco | es |
dc.date.accessioned | 2020-05-06T09:09:00Z | |
dc.date.available | 2020-05-06T09:09:00Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Araujo Garrido, J.L., Baisón Olmo, F., Bernal Bayard, J., Romero Portillo, F. y Ramos Morales, F. (2020). Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1. International Journal of Molecular Sciences, 21 (9), 1-18. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/96183 | |
dc.description.abstract | Salmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase activity. We carried out a yeast two-hybrid screen and have identified tubulin-binding cofactor B (TBCB) as a new binding partner for this effector. SseK1 catalyzed the addition of N-acetylglucosamine to arginine on TBCB, and its expression promoted the stabilization of the microtubule cytoskeleton of HEK293T cells. The conserved Asp-x-Asp (DxD) motif that is essential for the activity of SseK1 was required for the binding and modification of TBCB and for the effect on the cytoskeleton. Our study has identified a novel target for SseK1 and suggests that this effector may have a role in the manipulation of the host cell microtubule network to provide a safe niche for this pathogen. | es |
dc.description.sponsorship | España Ministerio de Economía, Industria y Competitividad – Agencia Estatal de Investigación, and the European Regional Development Fund, grant number SAF2016-75365-R; | es |
dc.description.sponsorship | European Union’s Horizon 2020 grant agreement No 842629. | es |
dc.format | application/pdf | es |
dc.format.extent | 18 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | International Journal of Molecular Sciences, 21 (9), 1-18. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Salmonella | es |
dc.subject | SseK1 | es |
dc.subject | TBCB | es |
dc.subject | arginine N-glycosyltransferase | es |
dc.subject | tubulin | es |
dc.subject | type III secretion | es |
dc.title | Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | SAF2016-75365-R; | es |
dc.relation.projectID | grant agreement No 842629 | es |
dc.relation.publisherversion | https://doi.org/10.3390/ijms21093193 | es |
dc.identifier.doi | 10.3390/ijms21093193 | es |
dc.journaltitle | International Journal of Molecular Sciences | es |
dc.publication.volumen | 21 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 18 | es |
dc.contributor.funder | Ministerio de Economia, Industria y Competitividad (MINECO). España | es |
dc.contributor.funder | European Regional Development Fund | es |
dc.contributor.funder | European Union (UE). H2020 | es |