Artículo
Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.)
Autor/es | Mapelli Brahm, Ana
Sánchez Rodriguez, Rosario Pan, Xue Moreno Pérez, Antonio Javier Garcés, Rafael Martínez Force, Enrique Weselake, Randall J. Salas Liñán, Joaquín Jesús Venegas Calerón, Mónica |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2020 |
Fecha de depósito | 2020-05-04 |
Publicado en |
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Resumen | Lysophosphatidylcholine acyltransferase (LPCAT, EC 2.3.1.23) is an evolutionarily conserved key enzyme in the Lands cycle that catalyzes acylation of lysophosphatidylcholine (LPC) to produce phosphatidylcholine (PC), the ... Lysophosphatidylcholine acyltransferase (LPCAT, EC 2.3.1.23) is an evolutionarily conserved key enzyme in the Lands cycle that catalyzes acylation of lysophosphatidylcholine (LPC) to produce phosphatidylcholine (PC), the main phospholipid in cellular membranes. In this study, three LPCAT genes from sunflower were identified and the corresponding proteins characterized. These HaLPCAT genes encoded functionally active enzymes that were able to complement a deficient yeast mutant. Moreover, enzymatic assays were carried out using microsomal preparations of the yeast cells. When acyl specificities were measured in the forward reaction, these enzymes exhibited a substrate preference for unsaturated acyl-CoAs, especially for linolenoyl-CoA, while in the reverse reaction, linoleoyl or linolenoyl acyl groups were transferred from PC to acyl-CoA to a similar extent. Expression levels of LPCAT genes were studied revealing distinct tissue-specific expression patterns. In summary, this study suggests that the combined forward and reverse reactions catalyzed by sunflower LPCATs facilitate acyl-exchange between the sn-2 position of PC and the acyl-CoA pool. Sunflower LPCATs displayed different characteristics, which could point to different functionalities, favoring the enrichment of seed triacylglycerols (TAGs) with polyunsaturated fatty acid (PUFA) |
Agencias financiadoras | Agencia Estatal de Investigación. España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
Identificador del proyecto | Project AGL2017- 83449-R. RW |
Cita | Mapelli Brahm, A., Sánchez Rodriguez, R., Pan, X., Moreno Pérez, A.J., Garcés, R., Martínez Force, E.,...,Venegas Calerón, M. (2020). Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Helianthus annuus L.). Frontiers in Plant Science, 11 (art. 403), 1-17. |
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