dc.creator | Albi Rodríguez, Tomás | es |
dc.creator | Ortiz Marchena, M Isabel | es |
dc.creator | Ruiz Pérez, Teresa | es |
dc.creator | Romero Rodríguez, José María | es |
dc.creator | Valverde Albacete, Federico | es |
dc.date.accessioned | 2019-02-06T08:07:38Z | |
dc.date.available | 2019-02-06T08:07:38Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Albi Rodríguez, T., Ortiz Marchena, M.I., Ruiz Pérez, T., Romero Rodríguez, J.M. y Valverde Albacete, F. (2014). Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity. Bio-protocol, 4 (23), 1-8. | |
dc.identifier.issn | 2331-8325 | es |
dc.identifier.uri | https://hdl.handle.net/11441/82556 | |
dc.description.abstract | Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014). | es |
dc.description.sponsorship | España,MINECO CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00 | es |
dc.description.sponsorship | España, Junta de Andalucía P06-CVI-01450 and P08-AGR-03582 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Bio-Protocol | es |
dc.relation.ispartof | Bio-protocol, 4 (23), 1-8. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Starch granule | es |
dc.subject | Starch purification | es |
dc.subject | GBSS activity | es |
dc.subject | Granule-bound starch synthase | es |
dc.title | Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | CSD2007-00057, BIO2008-02292, and BIO2011-28847-C02-00 | es |
dc.relation.projectID | P06-CVI-01450 and P08-AGR-03582 | es |
dc.relation.publisherversion | http://dx.doi.org/10.21769/BioProtoc.1316 | es |
dc.identifier.doi | 10.21769/BioProtoc.1316 | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Bio-protocol | es |
dc.publication.volumen | 4 | es |
dc.publication.issue | 23 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 8 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |
dc.contributor.funder | Junta de Andalucía | |