Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
|Author||Albi Rodríguez, Tomás
Ortiz Marchena, M Isabel
Ruiz Pérez, Teresa
Romero Rodríguez, José María
Valverde Albacete, Federico
|Department||Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular|
|Published in||Bio-protocol, 4 (23), 1-8.|
|Abstract||Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 126.96.36.199). ...
Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 188.8.131.52). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).
|Citation||Albi Rodríguez, T., Ortiz Marchena, M.I., Ruiz Pérez, T., Romero Rodríguez, J.M. y Valverde Albacete, F. (2014). Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity. Bio-protocol, 4 (23), 1-8.|
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