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dc.creatorCarrillo Jiménez, Alejandroes
dc.creatorPuigdellívol, Mares
dc.creatorVilalta, Annaes
dc.creatorVenero Recio, José Luises
dc.creatorBrown, Charles Guyes
dc.creatorBurguillos García, Miguel Ángeles
dc.date.accessioned2018-10-16T09:20:37Z
dc.date.available2018-10-16T09:20:37Z
dc.date.issued2018
dc.identifier.citationCarrillo Jiménez, A., Puigdellívol, M., Vilalta, ., Venero Recio, J.L., Brown, C.G. y Burguillos García, M.Á. (2018). Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation. Frontiers in Cellular Neuroscience, 12 (313), 1-8.
dc.identifier.issn1662-5102es
dc.identifier.urihttps://hdl.handle.net/11441/79435
dc.description.abstractMicroglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer’s disease (AD). The use of primary microglial cell cultures has proved to be a valuable tool to study microglial biology under various conditions. However, more advanced transfection methodologies for primary cultured microglia are still needed, as current methodologies provide low transfection efficiency and induce cell death and/or inflammatory activation of the microglia. Here, we describe an easy, and effective method based on the Glial-Mag method (OZ Biosciences) using magnetic nanoparticles and a magnet to successfully transfect primary microglia cells with different small interfering RNAs (siRNAs). This method does not require specialist facilities or specific training and does not induce cell toxicity or inflammatory activation. We demonstrate that this protocol successfully decreases the expression of two key genes associated with AD, the triggering receptor expressed in myeloid cells 2 (TREM2) and CD33, in primary microglia cell cultures.es
dc.description.sponsorshipEspaña, Ministerio de Educación, Cultura y Deporte a pre-doctoral fellowshipes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.ispartofFrontiers in Cellular Neuroscience, 12 (313), 1-8.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectmicrogliaes
dc.subjectsiRNAes
dc.subjecttransfectiones
dc.subjectTREM2es
dc.subjectCD33es
dc.subjectAlzheimer’s diseasees
dc.titleEffective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammationes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica y Biología Moleculares
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fncel.2018.00313es
dc.identifier.doi10.3389/fncel.2018.00313es
idus.format.extent8 p.es
dc.journaltitleFrontiers in Cellular Neurosciencees
dc.publication.volumen12es
dc.publication.issue313es
dc.publication.initialPage1es
dc.publication.endPage8es
dc.contributor.funderMinisterio de Educación, Cultura y Deporte (MECD). España

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Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional