dc.creator | Adam, Alexander | es |
dc.creator | Deimel, Stephan | es |
dc.creator | Pardo Medina, Javier | es |
dc.creator | García Martínez, Jorge | es |
dc.creator | Konte, Tilen | es |
dc.creator | Ávalos Cordero, Francisco Javier | es |
dc.creator | Limón Mirón, María del Carmen | es |
dc.date.accessioned | 2018-02-20T09:21:06Z | |
dc.date.available | 2018-02-20T09:21:06Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Adam, A., Deimel, S., Pardo Medina, J., García Martínez, J., Konte, T., Ávalos Cordero, F.J. y Limón Mirón, M.d.C. (2018). Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction. International Journal of Molecular Sciences, 19 (1), 215-. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/70425 | |
dc.description.abstract | Fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusarium fujikuroi exhibits two different rhodopsins, CarO and OpsA. CarO was previously characterized as a light-driven proton pump. We further analyzed the pumping behavior of CarO by patch-clamp experiments. Our data show that CarO pumping activity is strongly augmented in the presence of the plant hormone indole-3-acetic acid and in sodium acetate, in a dose-dependent manner under slightly acidic conditions. By contrast, under these and other tested conditions, the Neurospora rhodopsin (NR)-like rhodopsin OpsA did not exhibit any pump activity. Basic local alignment search tool (BLAST) searches in the genomes of ascomycetes revealed the occurrence of rhodopsin-encoding genes mainly in phyto-associated or phytopathogenic fungi, suggesting a possible correlation of the presence of rhodopsins with fungal ecology. In accordance, rice plants infected with a CarO-deficient F. fujikuroi strain showed more severe bakanae symptoms than the reference strain, indicating a potential role of the CarO rhodopsin in the regulation of plant infection by this fungus. | es |
dc.description.sponsorship | España Ministerio de Ciencia y tecnología BIO20010-15430 | es |
dc.description.sponsorship | Junta de Andalucía CTS-6638 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | International Journal of Molecular Sciences, 19 (1), 215-. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | fungal rhodopsins | es |
dc.subject | CarO | es |
dc.subject | OpsA | es |
dc.subject | Fusarium fujikuroi | es |
dc.subject | Oryza sativa | es |
dc.subject | rice–plant infection | es |
dc.subject | green light perception | es |
dc.subject | indole-3-acetic acid (IAA) | es |
dc.subject | bakanae | es |
dc.subject | patch-clamp | es |
dc.title | Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BIO20010-15430 | es |
dc.relation.projectID | CTS-6638 | es |
dc.relation.publisherversion | http://dx.doi.org/10.3390/ijms19010215 | es |
dc.identifier.doi | 10.3390/ijms19010215 | es |
dc.journaltitle | International Journal of Molecular Sciences | es |
dc.publication.volumen | 19 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 215 | es |
dc.contributor.funder | Ministerio de Ciencia y Tecnología (MCYT). España | |
dc.contributor.funder | Junta de Andalucía | |