Mostrar el registro sencillo del ítem

Artículo

dc.creatorGallastegui, Edurnees
dc.creatorMillán Zambrano, Gonzaloes
dc.creatorTerme, Jean Micheles
dc.creatorChávez de Diego, Sebastiánes
dc.creatorJordan, Albertes
dc.date.accessioned2017-12-04T19:23:44Z
dc.date.available2017-12-04T19:23:44Z
dc.date.issued2011
dc.identifier.citationGallastegui, E., Millán Zambrano, G., Terme, J.M., Chávez de Diego, S. y Jordan, A. (2011). Chromatin reassembly factors are involved in transcriptional interference promoting HIV latency. Journal of Virology, 85 (7), 3187-3202.
dc.identifier.issn0022-538X (impreso)es
dc.identifier.issn1098-5514 (electrónico)es
dc.identifier.urihttp://hdl.handle.net/11441/67234
dc.description.abstractThe establishment of a stable reservoir of latently infected cells allows HIV to persist in the host. Usually, HIV infection of T cells results in integration of the viral genome, with a preference for regions in the human genome containing active genes, viral expression, and production of new viruses. However, in rare cases T cells become latently infected, and this is presumed to be due to a combination of two factors: integrated viruses are not efficiently transcribed and infected T cells revert to a resting memory state. HIV latency has been associated with provirus integration in regions of constitutive heterochromatin, gene deserts, or very highly expressed genes. We have investigated the transcriptional consequences of latent HIV integration into cellular genes and the involvement of chromatin reassembly factors (CRFs) in the transcriptional interference that a host gene exerts on the integrated cryptic HIV promoter. Chimeric transcripts containing sequences from the host gene and HIV can be detected, having been initiated at promoters of either the cell or the virus. Reactivation of HIV downregulates host gene expression. Cryptic promoters might remain inactive due to the repressive chromatin configuration established by CRFs during transcription elongation. Depletion of CRFs such as Spt6, Chd1, and FACT, or the histone chaperones ASF1a and HIRA, promoted HIV reactivation, concomitantly with chromatin relaxation and a decrease in general RNA polymerase activity. Overall, our results indicate that CRFs play a role in maintaining HIV latency by transcriptional interference when the provirus is integrated into an intron of a highly active gene.es
dc.description.sponsorshipFundación para la Investigación y Prevencion del SIDA FIPSE 36602/06es
dc.description.sponsorshipInstituto de Salud Carlos III PI05/1831es
dc.description.sponsorshipMinisterio de Ciencia e Innovación BFU2008-00359/BMCes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofJournal of Virology, 85 (7), 3187-3202.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleChromatin reassembly factors are involved in transcriptional interference promoting HIV latencyes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDFIPSE 36602/06es
dc.relation.projectIDPI05/1831es
dc.relation.projectIDBFU2008-00359/BMCes
dc.relation.publisherversionhttp://dx.doi.org/10.1128/JVI.01920-10es
dc.identifier.doi10.1128/JVI.01920-10es
idus.format.extent16 p.es
dc.journaltitleJournal of Virologyes
dc.publication.volumen85es
dc.publication.issue7es
dc.publication.initialPage3187es
dc.publication.endPage3202es
dc.identifier.sisius20090661es

FicherosTamañoFormatoVerDescripción
Chromatin Reassembly.pdf2.335MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional