dc.creator | Mischo, Hannah E. | es |
dc.creator | Gómez González, Belén | es |
dc.creator | Grzechnik, Pawel | es |
dc.creator | García Rondón, Ana | es |
dc.creator | Wei, Wu | |
dc.creator | Steinmetz, Lars | |
dc.creator | Aguilera López, Andrés | |
dc.creator | Proudfoot, Nick J. | |
dc.date.accessioned | 2017-11-22T18:06:16Z | |
dc.date.available | 2017-11-22T18:06:16Z | |
dc.date.issued | 2011-01-06 | |
dc.identifier.citation | Mischo, H.E., Gómez González, B., Grzechnik, P., García Rondón, A., Wei, W., Steinmetz, L.,...,Proudfoot, N.J. (2011). Yeast Sen1 helicase protects the genome from transcription-associated instability. Molecular Cell, 41 (1), 21-32. | |
dc.identifier.issn | 1097-2765 (impreso) | es |
dc.identifier.uri | http://hdl.handle.net/11441/66490 | |
dc.description.abstract | Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structures. Loss of Sen1 results in transient R loop accumulation and so elicits transcription-associated recombination. SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación BFU2006-05260, CSD2007-0015 | es |
dc.description.sponsorship | Junta de Andalucía BIO102, CV2549 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Molecular Cell, 41 (1), 21-32. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Yeast Sen1 helicase protects the genome from transcription-associated instability | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BFU2006-05260 | es |
dc.relation.projectID | CSD2007-0015 | es |
dc.relation.projectID | BIO102 | es |
dc.relation.projectID | CV2549 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.molcel.2010.12.007 | es |
dc.identifier.doi | 10.1016/j.molcel.2010.12.007 | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | Molecular Cell | es |
dc.publication.volumen | 41 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 21 | es |
dc.publication.endPage | 32 | es |
dc.identifier.sisius | 6557812 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | |
dc.contributor.funder | Junta de Andalucía | |