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dc.creatorNúñez Hernández, Cristinaes
dc.creatorTierrez, Albertoes
dc.creatorD. Ortega, Álvaroes
dc.creatorPucciarelli, María Gracielaes
dc.creatorGodoy, Martaes
dc.creatorEisman, Blancaes
dc.creatorCasadesús Pursals, Josepes
dc.creatorGarcía del Portillo, Franciscoes
dc.date.accessioned2017-07-18T15:44:18Z
dc.date.available2017-07-18T15:44:18Z
dc.date.issued2013-01
dc.identifier.citationNúñez Hernández, C., Tierrez, A., D. Ortega, Á., Pucciarelli, M.G., Godoy, M., Eisman, B.,...,García del Portillo, F. (2013). Genome expression analysis of nonproliferating intracellular Salmonella enterica serovar ty phimurium unravels an acid pH-dependent PhoP-PhoQ response essential for dormancy. Infection and Immunity, 81 (1), 154-165.
dc.identifier.issn0019-9567 (impreso)es
dc.identifier.issn1098-5522 (electronico)es
dc.identifier.urihttp://hdl.handle.net/11441/62639
dc.description.abstractGenome-wide expression analyses have provided clues on how Salmonella proliferates inside cultured macrophages and epithelial cells. However, in vivo studies show that Salmonella does not replicate massively within host cells, leaving the underlyingmechanisms of such growth control largely undefined. In vitro infection models based on fibroblasts or dendritic cells reveal limited proliferation of the pathogen, but it is presently unknown whether these phenomena reflect events occurring in vivo. Fibroblasts are distinctive, since they represent a nonphagocytic cell type in which S. enterica serovar Typhimurium actively attenuates intracellular growth. Here, we show in the mouse model that S. Typhimurium restrains intracellular growth within nonphagocytic cells positioned in the intestinal lamina propria. This response requires a functional PhoP-PhoQ system and is reproduced inprimary fibroblasts isolated from the mouse intestine. The fibroblast infection model was exploited to generate transcriptome data, which revealed that [H11011]2% (98 genes) of the S. Typhimurium genome is differentially expressed in nongrowing intracellular bacteria. Changes include metabolic reprogramming to microaerophilic conditions, induction of virulence plasmid genes,upregulation of the pathogenicity islands SPI-1 and SPI-2, and shutdown of flagella production and chemotaxis. Comparison of relative protein levels of several PhoP-PhoQ-regulated functions (PagN, PagP, and VirK) in nongrowing intra-cellular bacteria andextracellular bacteria exposed to diverse PhoP-PhoQ-inducing signals denoted a regulation responding to acidic pH. These data demonstrate that S. Typhimurium restrains intracellular growth in vivo and support a model in which dormant intracellular bacteriacould sense vacuolar acidification to stimulate the PhoP-PhoQ system for preventing intracellular overgrowth.es
dc.description.sponsorshipMinisterio de Economía y Competitividad BIO2010-18885 CSD2008-00013-INTERMODS BIO2010-15023es
dc.description.sponsorshipJunta de Andalucía P10-CVI-5879es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofInfection and Immunity, 81 (1), 154-165.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleGenome expression analysis of nonproliferating intracellular Salmonella enterica serovar ty phimurium unravels an acid pH-dependent PhoP-PhoQ response essential for dormancyes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/ BIO2010-18885es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/CSD2008-00013es
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/BIO2010-15023es
dc.relation.publisherversionhttp://dx.doi.org/10.1128/IAI.01080-12es
dc.identifier.doi10.1128/IAI.01080-12es
idus.format.extent12 p.es
dc.journaltitleInfection and Immunityes
dc.publication.volumen81es
dc.publication.issue1es
dc.publication.initialPage154es
dc.publication.endPage165es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España
dc.contributor.funderJunta de Andalucía

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