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dc.creatorWellinger, Ralf Erikes
dc.creatorPrado Velasco, José Félixes
dc.creatorAguilera López, Andréses
dc.date.accessioned2017-07-04T10:35:19Z
dc.date.available2017-07-04T10:35:19Z
dc.date.issued2006
dc.identifier.citationWellinger, R.E., Prado, F. y Aguilera López, A. (2006). Replication fork progression is impaired by transcription in hyperrecombinant yeast cells lacking a functional THO complex. Molecular and Cellular Biology, 26 (8), 3327-3334.
dc.identifier.issn0270-7306es
dc.identifier.urihttp://hdl.handle.net/11441/61928
dc.description.abstractTHO/TREX is a conserved, eukaryotic protein complex operating at the interface between transcription and messenger ribonucleoprotein (mRNP) metabolism. THO mutations impair transcription and lead to increased transcription-associated recombination (TAR). These phenotypes are dependent on the nascent mRNA; however, the molecular mechanism by which impaired mRNP biogenesis triggers recombination in THO/TREX mutants is unknown. In this study, we provide evidence that deficient mRNP biogenesis causes slowdown or pausing of the replication fork in hpr1 mutants. Impaired replication appears to depend on sequence-specific features since it was observed upon activation of lacZ but not leu2 transcription. Replication fork progression could be partially restored by hammerhead ribozyme-guided self-cleavage of the nascent mRNA. Additionally, hpr1 increased the number of S-phase but not G2-dependent TAR events as well as the number of budded cells containing Rad52 repair foci. Our results link transcription-dependent genomic instability in THO mutants with impaired replication fork progression, suggesting a molecular basis for a connection between inefficient mRNP biogenesis and genetic instabilityes
dc.description.sponsorshipMinisterio de Educación y Ciencia SAF2003-00204es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofMolecular and Cellular Biology, 26 (8), 3327-3334.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleReplication fork progression is impaired by transcription in hyperrecombinant yeast cells lacking a functional THO complexes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDSAF2003-00204es
dc.relation.publisherversion10.1128/MCB.26.8.3327–3334.2006es
dc.identifier.doi10.1128/MCB.26.8.3327–3334.2006es
idus.format.extent7 p.es
dc.journaltitleMolecular and Cellular Biologyes
dc.publication.volumen26es
dc.publication.issue8es
dc.publication.initialPage3327es
dc.publication.endPage3334es
dc.contributor.funderMinisterio de Educación y Ciencia (MEC). España

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