dc.creator | Cota García, Ignacio | es |
dc.creator | Blanc Potard, Anne Béatrice | es |
dc.creator | Casadesús Pursals, Josep | es |
dc.date.accessioned | 2017-06-09T09:52:50Z | |
dc.date.available | 2017-06-09T09:52:50Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Cota García, I., Blanc Potard, A.B. y Casadesús Pursals, J. (2012). STM2209-STM2208 (opvAB): A Phase Variation Locus of Salmonella enterica Involved in Control of O-Antigen Chain Length. PLoS One, 7 (5), e36863.1-e36863.13. | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | http://hdl.handle.net/11441/61174 | |
dc.description.abstract | STM2209 and STM2208 are contiguous loci annotated as putative protein-coding genes in the chromosome of Salmonella enterica. Lack of homologs in related Enterobacteria and low G+C content suggest that S. enterica may have acquired STM2209-STM2208 by horizontal transfer. STM2209 and STM2208 are co-transcribed from a promoter upstream STM2209, and their products are inner (cytoplasmic) membrane proteins. Analysis with the bacterial adenylate cyclase two-hybrid system suggests that STM2209 and STM2208 may interact. Expression of STM2209-STM2208 is subjected to phase variation in wild type Salmonella enterica serovar Typhimurium. Switching frequencies in LB medium are 6.1×10−5 (OFF→ON) and 3.7×10−2 (ON→OFF) per cell and generation. Lack of DNA adenine methylation locks STM2209-STM2208 in the ON state, and lack of the LysR-type factor OxyR locks STM2209-STM2208 in the OFF state. OxyR-dependent activation of STM2209-STM2208 expression is independent of the oxidation state of OxyR. Salmonella cultures locked in the ON state show alteration of O-antigen length in the lipopolysaccharide, reduced absorption of bacteriophage P22, impaired resistance to serum, and reduced proliferation in macrophages. Phenotypic heterogeneity generated by STM2209-STM2208 phase variation may thus provide defense against phages. In turn, formation of a subpopulation unable to proliferate in macrophages may restrain Salmonella spread in animal organs, potentially contributing to successful infection. | es |
dc.description.sponsorship | España, Ministerio de Ciencia e Innovación BIO2007-67457-CO2-02 and CSD2008-00013 | es |
dc.description.sponsorship | Junta de Andalucía, Consejería de Innovación, Ciencia y Empresa CVI-5879 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS One, 7 (5), e36863.1-e36863.13. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | STM2209-STM2208 (opvAB): A Phase Variation Locus of Salmonella enterica Involved in Control of O-Antigen Chain Length | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | BIO2007-67457-CO2-02 and CSD2008-00013 | es |
dc.relation.projectID | CVI-5879 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0036863 | es |
dc.identifier.doi | 10.1371/journal.pone.0036863 | es |
idus.format.extent | 13 p. | es |
dc.journaltitle | PLoS One | es |
dc.publication.volumen | 7 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | e36863.1 | es |
dc.publication.endPage | e36863.13 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | |
dc.contributor.funder | Junta de Andalucía | |