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dc.creatorGómez Herreros, Fernandoes
dc.creatorMiguel Jiménez, María Dolores dees
dc.creatorMorillo Huesca, Macarenaes
dc.creatorDelgado Ramos, Lidiaes
dc.creatorMuñoz Centeno, María de la Cruzes
dc.creatorChávez de Diego, Sebastiánes
dc.date.accessioned2017-03-10T13:23:07Z
dc.date.available2017-03-10T13:23:07Z
dc.date.issued2012
dc.identifier.citationGómez Herreros, F., Miguel Jiménez, M.D.D., Morillo Huesca, M., Delgado Ramos, L., Muñoz Centeno, M.d.l.C. y Chávez de Diego, S. (2012). TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress. Nucleic Acids Research, 40 (14), 6508-6519.
dc.identifier.issn0305-1048es
dc.identifier.urihttp://hdl.handle.net/11441/55720
dc.description.abstractTranscription factor IIS (TFIIS) stimulates RNA cleavage by RNA polymerase II by allowing backtracked enzymes to resume transcription elongation. Yeast cells do not require TFIIS for viability, unless they suffer severe transcriptional stress due to NTP-depleting drugs like 6-azauracil or mycophenolic acid. In order to broaden our knowledge on the role of TFIIS under transcriptional stress, we carried out a genetic screening for suppressors of TFIIS-lacking cells’ sensitivity to 6-azauracil and mycophenolic acid. Five suppressors were identified, four of which were related to the transcriptional regulation of those genes encoding ribosomal components [rRNAs and ribosomal proteins (RP)], including global regulator SFP1. This led us to discover that RNA polymerase II is hypersensitive to the absence of TFIIS under NTP scarcity conditions when transcribing RP genes. The absence of Sfp1 led to a profound alteration of the transcriptional response to NTP-depletion, thus allowing the expression of RP genes to resist these stressful conditions in the absence of TFIIS. We discuss the effect of transcriptional stress on ribosome biogenesis and propose that TFIIS contributes to prevent a transcriptional imbalance between rDNA and RP genes.es
dc.description.sponsorshipEspaña Ministerio de Economía y competitividad BFU2007-67575-C03-02es
dc.description.sponsorshipEspaña Ministerio de Economía y competitividad BFU-2010-21975-C03-03es
dc.description.sponsorshipJunta de Andalucía P07-CVI-02623es
dc.description.sponsorshipJunta de Andalucía P08-CVI-0350es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofNucleic Acids Research, 40 (14), 6508-6519.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleTFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stresses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDBFU2007-67575-C03-02es
dc.relation.projectIDBFU-2010-21975-C03-03es
dc.relation.projectIDP08-CVI-0350es
dc.relation.projectIDP07-CVI-02623es
dc.relation.publisherversionhttp://dx.doi.org/ 10.1093/nar/gks340es
dc.identifier.doi10.1093/nar/gks340es
idus.format.extent11 p.es
dc.journaltitleNucleic Acids Researches
dc.publication.volumen40es
dc.publication.issue14es
dc.publication.initialPage6508es
dc.publication.endPage6519es

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