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dc.creatorAcosta Jurado, Sebastiánes
dc.creatorNavarro Gómez, Pilares
dc.creatorMurdoch, Piedad del Socorroes
dc.creatorCrespo Rivas, Juan Carloses
dc.creatorShi Jiees
dc.creatorCuesta Berrio, Lidiaes
dc.creatorRuiz Sainz, José Enriquees
dc.creatorRodríguez Carvajal, Miguel Ángeles
dc.creatorVinardell González, José Maríaes
dc.date.accessioned2017-03-08T14:17:30Z
dc.date.available2017-03-08T14:17:30Z
dc.date.issued2016
dc.identifier.citationAcosta Jurado, S., Navarro Gómez, P., Murdoch, P.d.S., Crespo Rivas, J.C., Shi Jie, , Cuesta Berrio; Lidia, ,...,Vinardell González, J.M. (2016). Exopolysaccharide Production by Sinorhizobium fredii HH103 Is Repressed by Genistein in a NodD1-Dependent Manner. Plos One, 11 (8), e0160499-1-e0160499-16.
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/11441/55586
dc.description.abstractIn the rhizobia-legume symbiotic interaction, bacterial surface polysaccharides, such as exopolysaccharide (EPS), lipopolysaccharide (LPS), K-antigen polysaccharide (KPS) or cyclic glucans (CG), appear to play crucial roles either acting as signals required for the progression of the interaction and/or preventing host defence mechanisms. The symbiotic significance of each of these polysaccharides varies depending on the specific rhizobialegume couple. In this work we show that the production of exopolysaccharide by Sinorhizobium fredii HH103, but not by other S. fredii strains such as USDA257 or NGR234, is repressed by nod gene inducing flavonoids such as genistein and that this repression is dependent on the presence of a functional NodD1 protein. In agreement with the importance of EPS for bacterial biofilms, this reduced EPS production upon treatment with flavonoids correlates with decreased biofilm formation ability. By using quantitative RT-PCR analysis we show that expression of the exoY2 and exoK genes is repressed in late stationary cultures of S. fredii HH103 upon treatment with genistein. Results presented in this work show that in S. fredii HH103 EPS production is regulated just in the opposite way than other bacterial signals such as Nod factors and type 3 secreted effectors: it is repressed by flavonoids and NodD1 and enhanced by the nod repressor NolR. These results are in agreement with our previous observations showing that lack of EPS production by S. fredii HH103 is not only non-detrimental but even beneficial for symbiosis with soybeanes
dc.description.sponsorshipMinisterio de Ciencia e Investigación BIO2011-30229-C02-01es
dc.description.sponsorshipJunta de Andalucía P11-CVI-7500es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPlos One, 11 (8), e0160499-1-e0160499-16.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBacterial polysaccharidees
dc.subjectExopolysaccharidees
dc.subjectFlavonoides
dc.subjectGenisteines
dc.subjectNodD1 proteines
dc.subjectPeptides and proteinses
dc.titleExopolysaccharide Production by Sinorhizobium fredii HH103 Is Repressed by Genistein in a NodD1-Dependent Manneres
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Química orgánicaes
dc.relation.projectIDBIO2011-30229-C02-01es
dc.relation.projectIDP11-CVI-7500es
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0160499es
dc.identifier.doi10.1371/journal.pone.0160499es
idus.format.extent16 p.es
dc.journaltitlePlos Onees
dc.publication.volumen11es
dc.publication.issue8es
dc.publication.initialPagee0160499-1es
dc.publication.endPagee0160499-16es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). España
dc.contributor.funderJunta de Andalucía

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