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dc.creatorPelechano, Vicentes
dc.creatorJimeno González, Silviaes
dc.creatorRodríguez Gil, Alfonsoes
dc.creatorGarcía Martínez, Josées
dc.creatorPérez Ortín, José E.es
dc.creatorChávez de Diego, Sebastiánes
dc.date.accessioned2017-03-08T14:16:03Z
dc.date.available2017-03-08T14:16:03Z
dc.date.issued2009
dc.identifier.citationPelechano, V., Jimeno González, S., Rodríguez Gil, A., García Martínez, ., Pérez Ortín, .E. y Chávez de Diego, S. (2009). Regulon-Specific Control of Transcription Elongation across the Yeast Genome. PLOS Genetics, 5 (8), e1000614.
dc.identifier.issn1553-7390es
dc.identifier.urihttp://hdl.handle.net/11441/55585
dc.description.abstractTranscription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classes, like those related to ribosomes and mitochondria. This proportion also responds to regulatory stimuli mediated by protein kinase A and, in relation to cytosolic ribosomal-protein genes, it is mediated by the silencing domain of Rap1. We found that this inactive form of RNA polymerase II, which accumulates along the full length of ribosomal protein genes, is phosphorylated in the Ser5 residue of the CTD, but is hypophosphorylated in Ser2. Using the same experimental approach, we show that the in vivo–depletion of FACT, a chromatin-related elongation factor, also produces a regulon-specific effect on the expression of the yeast genome. This work demonstrates that the regulation of transcription elongation is a widespread, gene class–dependent phenomenon that also affects housekeeping genes.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPLOS Genetics, 5 (8), e1000614.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleRegulon-Specific Control of Transcription Elongation across the Yeast Genomees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversionhttp://dx.doi.org/ 10.1371/journal.pgen.1000614es
dc.identifier.doi10.1371/journal.pgen.1000614es
idus.format.extent13 p.es
dc.journaltitlePLOS Geneticses
dc.publication.volumen5es
dc.publication.issue8es
dc.publication.initialPagee1000614es

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