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Artículo
Roles of human POLD1 and POLD3 in genome stability
dc.creator | Tumini, Emanuela | es |
dc.creator | Barroso Ceballos, Sonia Inés | es |
dc.creator | Pérez Calero, Carmen | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2017-02-27T10:17:25Z | |
dc.date.available | 2017-02-27T10:17:25Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Tumini, E., Barroso Ceballos, S.I., Pérez Calero, C. y Aguilera López, A. (2016). Roles of human POLD1 and POLD3 in genome stability. Scientific Reports, 6, 1-13. | |
dc.identifier.issn | 2045-2322 | es |
dc.identifier.uri | http://hdl.handle.net/11441/54890 | |
dc.description.abstract | DNA replication is essential for cellular proliferation. If improperly controlled it can constitute a major source of genome instability, frequently associated with cancer and aging. POLD1 is the catalytic subunit and POLD3 is an accessory subunit of the replicative Pol δ polymerase, which also functions in DNA repair, as well as the translesion synthesis polymerase Pol ζ, whose catalytic subunit is REV3L. In cells depleted of POLD1 or POLD3 we found a differential but general increase in genome instability as manifested by DNA breaks, S-phase progression impairment and chromosome abnormalities. Importantly, we showed that both proteins are needed to maintain the proper amount of active replication origins and that POLD3-depletion causes anaphase bridges accumulation. In addition, POLD3-associated DNA damage showed to be dependent on RNA-DNA hybrids pointing toward an additional and specific role of this subunit in genome stability. Interestingly, a similar increase in RNA-DNA hybrids-dependent genome instability was observed in REV3L-depleted cells. Our findings demonstrate a key role of POLD1 and POLD3 in genome stability and S-phase progression revealing RNA-DNA hybrids-dependent effects for POLD3 that might be partly due to its Pol ζ interaction. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Nature | es |
dc.relation.ispartof | Scientific Reports, 6, 1-13. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Roles of human POLD1 and POLD3 in genome stability | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1038/srep38873 | es |
dc.identifier.doi | 10.1038/srep38873 | es |
idus.format.extent | 14 p. | es |
dc.journaltitle | Scientific Reports | es |
dc.publication.volumen | 6 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 13 | es |
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pubmed10srep38873.pdf | 2.474Mb | ![]() | Ver/ | |