dc.creator | Kressler, Dieter | es |
dc.creator | Cruz Díaz, Jesús de la | es |
dc.creator | Rojo, Manuel | es |
dc.creator | Linder, Patrick | es |
dc.date.accessioned | 2016-08-08T11:10:27Z | |
dc.date.available | 2016-08-08T11:10:27Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | Kressler, D., Cruz Díaz, J.d.l., Rojo, M. y Linder, P. (1997). Fal1p is an essential DEAD-box protein involved in 40S-ribosomal- subunit biogenesis in saccharomyces cerevisiae. Molecular and Cellular Biology, 17 (12), 7283-7294. | |
dc.identifier.issn | 0270-7306 | es |
dc.identifier.uri | http://hdl.handle.net/11441/44290 | |
dc.description.abstract | A previously uncharacterizedSaccharomyces cerevisiaegene,FAL1, was found by sequence comparison as a
homolog of the eukaryotic translation initiation factor 4A (eIF4A). Fal1p has 55% identity and 73% similarity
on the amino acid level to yeast eIF4A, the prototype of ATP-dependent RNA helicases of the DEAD-box protein
family. Although clearly grouped in the eIF4A subfamily, the essential Fal1p displays a different subcellular
function and localization. An HA epitope-tagged Fal1p is localized predominantly in the nucleolus. Polysome
analyses in a temperature-sensitivefal1-1mutant and a Fal1p-depleted strain reveal a decrease in the number
of 40S ribosomal subunits. Furthermore, these strains are hypersensitive to the aminoglycoside antibiotics
paromomycin and neomycin. Pulse-chase labeling of pre-rRNA and steady-state-level analysis of pre-rRNAs
and mature rRNAs by Northern hybridization and primer extension in the Fal1p-depleted strain show that
Fal1p is required for pre-rRNA processing at sites A0,A1, and A
2. Consequently, depletion of Fal1p leads to
decreased 18S rRNA levels and to an overall deficit in 40S ribosomal subunits. Together, these results
implicate Fal1p in the 18S rRNA maturation pathway rather than in translation initiation | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Molecular and Cellular Biology, 17 (12), 7283-7294. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Fal1p is an essential DEAD-box protein involved in 40S-ribosomal- subunit biogenesis in saccharomyces cerevisiae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://mcb.asm.org/content/17/12/7283.full.pdf+html | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | Molecular and Cellular Biology | es |
dc.publication.volumen | 17 | es |
dc.publication.issue | 12 | es |
dc.publication.initialPage | 7283 | es |
dc.publication.endPage | 7294 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/44290 | |