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dc.creatorKressler, Dieteres
dc.creatorCruz Díaz, Jesús de laes
dc.creatorRojo, Manueles
dc.creatorLinder, Patrickes
dc.date.accessioned2016-08-08T11:10:27Z
dc.date.available2016-08-08T11:10:27Z
dc.date.issued1997
dc.identifier.citationKressler, D., Cruz Díaz, J.d.l., Rojo, M. y Linder, P. (1997). Fal1p is an essential DEAD-box protein involved in 40S-ribosomal- subunit biogenesis in saccharomyces cerevisiae. Molecular and Cellular Biology, 17 (12), 7283-7294.
dc.identifier.issn0270-7306es
dc.identifier.urihttp://hdl.handle.net/11441/44290
dc.description.abstractA previously uncharacterizedSaccharomyces cerevisiaegene,FAL1, was found by sequence comparison as a homolog of the eukaryotic translation initiation factor 4A (eIF4A). Fal1p has 55% identity and 73% similarity on the amino acid level to yeast eIF4A, the prototype of ATP-dependent RNA helicases of the DEAD-box protein family. Although clearly grouped in the eIF4A subfamily, the essential Fal1p displays a different subcellular function and localization. An HA epitope-tagged Fal1p is localized predominantly in the nucleolus. Polysome analyses in a temperature-sensitivefal1-1mutant and a Fal1p-depleted strain reveal a decrease in the number of 40S ribosomal subunits. Furthermore, these strains are hypersensitive to the aminoglycoside antibiotics paromomycin and neomycin. Pulse-chase labeling of pre-rRNA and steady-state-level analysis of pre-rRNAs and mature rRNAs by Northern hybridization and primer extension in the Fal1p-depleted strain show that Fal1p is required for pre-rRNA processing at sites A0,A1, and A 2. Consequently, depletion of Fal1p leads to decreased 18S rRNA levels and to an overall deficit in 40S ribosomal subunits. Together, these results implicate Fal1p in the 18S rRNA maturation pathway rather than in translation initiationes
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofMolecular and Cellular Biology, 17 (12), 7283-7294.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleFal1p is an essential DEAD-box protein involved in 40S-ribosomal- subunit biogenesis in saccharomyces cerevisiaees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversionhttp://mcb.asm.org/content/17/12/7283.full.pdf+htmles
idus.format.extent12 p.es
dc.journaltitleMolecular and Cellular Biologyes
dc.publication.volumen17es
dc.publication.issue12es
dc.publication.initialPage7283es
dc.publication.endPage7294es
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/44290

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