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Artículo
Genetic Variability among ampC Genes from Acinetobacter Genomic Species 3
dc.creator | Beceiro Casas, Alejandro | es |
dc.creator | Pérez, Astrid | es |
dc.creator | Fernández-Cuenca, Felipe | es |
dc.creator | Martínez-Martínez, Luis | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Vila, Jordi | es |
dc.creator | Rodríguez-Baño, Jesús | es |
dc.creator | Cisneros, José Miguel | es |
dc.creator | Pachón Díaz, Jerónimo | es |
dc.creator | Bou, Germán | es |
dc.date.accessioned | 2016-05-13T17:22:11Z | |
dc.date.available | 2016-05-13T17:22:11Z | |
dc.date.issued | 2009-03 | |
dc.identifier.issn | 0066-4804 | es |
dc.identifier.uri | http://hdl.handle.net/11441/41212 | |
dc.description.abstract | As a part of a nationwide study in Spain, 15 clinical isolates of Acinetobacter genomic species 3 (AG3) were analyzed. The main objective of the study was to characterize the ampC genes from these isolates and to determine their involvement in B-lactam resistance in AG3. The 15 AG3 isolates showed different profiles of resistance to ampicillin (range of MICs, 12 to >256 μg/ml). Nucleotide sequencing of the 15 ampC genes yielded 12 new AmpC enzymes (ADC-12 to ADC-23). The 12 AG3 enzymes showed 93.7 to 96.1% amino acid identity with respect to the AmpC enzyme from Acinetobacter baumannii (ADC-1 enzyme). Eight out of fifteen ampC genes were expressed in Escherichia coli cells under the control of a common promoter, and with the exception of one isolate (isolate 65, which showed lower B-lactam MICs), significant differences in overall B-lactam MICs for E. coli cells expressing AG3 ampC genes were not revealed. No significant differences in ampC gene expression in AG3 clinical isolates were revealed by reverse transcription-PCR analysis. A detailed analysis of the 12 AmpC protein sequences revealed that amino acid replacements (in comparison with those of ADC-1) occurred mainly in the same positions, although none were located in important functional domains such as the Ω- loop or conserved B-lactamase motifs. Kinetic experiments performed with three representative AmpC enzymes (ADC-14, -16, and -18) in some cases revealed dramatic changes in Km and kcat values for B-lactams. No ISAba1 was detected upstream of the ampC genes. Our results reveal 12 new ampC genes in AG3. The enzymes showed a moderate degree of variability, and they are tentatively named ADC-12 to ADC-23 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Antimicrobial Agents and Chemotherapy, 53(3), 1177–1184 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Genetic Variability among ampC Genes from Acinetobacter Genomic Species 3 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/aac.00485-08 | es |
dc.identifier.doi | http://dx.doi.org/10.1128/aac.00485-08 | es |
idus.format.extent | 8 p. | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/41212 |
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