dc.creator | Ortega, Adriana | es |
dc.creator | Oteo, Jesús | es |
dc.creator | Aranzamendi-Zaldumbide, Maitane | es |
dc.creator | Bartolomé, Rosa M. | es |
dc.creator | Bou, Germán | es |
dc.creator | Cercenado, Emilia | es |
dc.creator | Conejo Gonzalo, Mª Carmen | es |
dc.creator | González-López, Juan José | es |
dc.creator | Marín, Mercedes | es |
dc.creator | Martínez-Martínez, Luis | es |
dc.creator | Marino, María | es |
dc.creator | Ferran, Navarro | es |
dc.creator | Oliver, Antonio | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.creator | Rivera, Alba | es |
dc.creator | Rodríguez-Baño, Jesús | es |
dc.creator | Weber, Irene | es |
dc.creator | Aracil, Belén | es |
dc.creator | Campos, José | es |
dc.date.accessioned | 2016-04-28T15:14:43Z | |
dc.date.available | 2016-04-28T15:14:43Z | |
dc.date.issued | 2012-07 | |
dc.identifier.issn | 0066-4804 | es |
dc.identifier.uri | http://hdl.handle.net/11441/40585 | |
dc.description.abstract | We conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavu-lanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC>32/16 g/ml) were collected at each of theseven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperpro-duction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC(18.3%), and production of inhibitor-resistant TEM (IRT) (17.5%). The IRTs identified were TEM-40 (33.3%), TEM-30(28.9%), TEM-33 (11.1%), TEM-32 (4.4%), TEM-34 (4.4%), TEM-35 (2.2%), TEM-54 (2.2%), TEM-76 (2.2%), TEM-79(2.2%), and the new TEM-185 (8.8%). By PFGE, a high degree of genetic diversity was observed although two well-defined
clusters were detected in the OXA-1-producing isolates: the C1 cluster consisting of 19 phylogroup A/sequence type 88[ST88] isolates and the C2 cluster consisting of 19 phylogroup B2/ST131 isolates (16 of them producing CTX-M-15). Each of the clusters was detected in six different hospitals. In total, 21.8% of the isolates were serotype O25b/phylogroup B2
(O25b/B2). AMC resistance in E. coli is widespread in Spain at the hospital and community levels. A high prevalence of OXA-1 was found. Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131. Dissemination of IRTs was frequent, and the epidemic O25b/B2/ST131 clone carried many different
mechanisms of AMC resistance. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Antimicrobial Agents and Chemotherapy, 56(7), 3576-3581 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Combinación de amoxicilina clavulanato potásico | es |
dc.subject | Farmacorresistencia bacteriana | es |
dc.subject | Infecciones por Escherichia coli | es |
dc.subject | Epidemiología molecular | es |
dc.title | Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.publisherversion | http://aac.asm.org/content/56/7/3576.abstract | es |
dc.identifier.doi | http://dx.doi.org/10.1128/AAC.06393-11 | es |
idus.format.extent | 6 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40585 | |