dc.creator | Ramos Rodríguez, Cayo Juan | es |
dc.creator | López Calderón, Isabel | es |
dc.date.accessioned | 2016-04-21T10:29:00Z | |
dc.date.available | 2016-04-21T10:29:00Z | |
dc.date.issued | 1992 | |
dc.identifier.citation | Ramos Rodríguez, C.J. y López Calderón, I. (1992). Overproduction of threonine by Saccharomyces cerevisiae mutants resistant to hydroxynorvaline. Applied and Environmental Microbiology, 58 (5), 1677-1682. | es |
dc.identifier.issn | 1098-5336 | es |
dc.identifier.uri | http://hdl.handle.net/11441/40233 | |
dc.description.abstract | In
this
work,
we
isolated
and
characterized
mutants
that
overproduce
threonine
from
Saccharomyces
cerevisiae.
The
mutants
were
selected
for
resistance
to
the
threonine
analog
a-amino-13-hydroxynorvalerate
(hydroxynorvaline),
and,
of
these,
the
ones
able
to
excrete
threonine
to
the
medium
were
chosen.
The
mutant
strains
produce
between
15
and
30
times
more
threonine
than
the
wild
type
does,
and,
to
a
lesser
degree,
they
also
accumulate
isoleucine.
Genetic
and
biochemical
studies
have
revealed
that
the
threonine
overproduction
is,
in
all
cases
studied,
associated
with
the
presence
in
the
strain
of
a
HOM3
allele
coding
for
a
mutant
aspartate
kinase
that
is
totally
or
partially
insensitive
to
feedback
inhibition
by
threonine.
This
enzyme
seems,
therefore,
to
be
crucial
in
the
regulation
of
threonine
biosynthesis
in
S.
cerevisiae.
The
results
obtained
suggest
that
this
strategy
could
be
efficiently
applied
to
the
isolation
of
threonine-overproducing
strains
of
yeasts
other
than
S.
cerevisiae,
even
those
used
industrially. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Applied and Environmental Microbiology,58 (5), 1677-1682 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Overproduction of threonine by Saccharomyces cerevisiae mutants resistant to hydroxynorvaline | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC195657/pdf/aem00046-0273.pdf | es |
dc.journaltitle | Applied and Environmental Microbiology | es |
dc.publication.volumen | 58 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | 1677 | es |
dc.publication.endPage | 1682 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/40233 | |