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dc.creatorBlanco, Jorge
dc.creatorMora, Azucena
dc.creatorMamani, Rosalia
dc.creatorLópez, Cecilia
dc.creatorBlanco, Miguel
dc.creatorDahbi, Ghizlane
dc.creatorHerrera, Alexandra
dc.creatorMarzoa, Juan
dc.creatorFernández, Val
dc.creatorCruz, Fernando de la
dc.creatorMartínez-Martínez, Luis
dc.creatorAlonso, María Pilar
dc.creatorNicolas-Chanoine, Marie-Hélène
dc.creatorJohnson, James R.
dc.creatorJohnston, Brian
dc.creatorLópez Cerero, Lorena
dc.creatorPascual Hernández, Álvaro
dc.creatorRodríguez-Baño, Jesús
dc.date.accessioned2016-03-11T18:32:46Z
dc.date.available2016-03-11T18:32:46Z
dc.date.issued2013-10
dc.identifier.citationBlanco, J., Mora, A., Mamani, R., López, C., Blanco, M., Dahbi, G.,...,Rodríguez-Baño, J. (2013). Four main virotypes among extended-spectrum-β-lactamase-producing isolates of Escherichia coli O25b:H4-B2-ST131: bacterial, epidemiological, and clinical characteristics.
dc.identifier.issn0095-1137es
dc.identifier.urihttp://hdl.handle.net/11441/38447
dc.description.abstractA total of 1,021 extended-spectrum- -lactamase-producing Escherichia coli (ESBLEC) isolates obtained in 2006 during a Spanish national survey conducted in 44 hospitals were analyzed for the presence of the O25b:H4-B2-ST131 (sequence type 131) clonal group. Overall, 195 (19%) O25b-ST131 isolates were detected, with prevalence rates ranging from 0% to 52% per hospital. Molecular characterization of 130 representative O25b-ST131 isolates showed that 96 (74%) were positive for CTX-M-15, 15 (12%) for CTX-M-14, 9 (7%) for SHV-12, 6 (5%) for CTX-M-9, 5 (4%) for CTX-M-32, and 1 (0.7%) each for CTX-M-3 and the new ESBL enzyme CTX-M-103. The 130 O25b-ST131 isolates exhibited relatively high virulence scores (mean, 14.4 virulence genes). Although the virulence profiles of the O25b-ST131 isolates were fairly homogeneous, they could be classified into four main virotypes based on the presence or absence of four distinctive virulence genes: virotypes A (22%) (afa FM955459 positive, iroN negative, ibeA negative, sat positive or negative), B (31%) (afa FM955459 negative, iroN positive, ibeA negative, sat positive or negative), C (32%) (afa FM955459 negative, iroN negative, ibeA negative, sat positive), and D (13%) (afa FM955459 negative, iroN positive or negative, ibeA positive, sat positive or negative). The four virotypes were also identified in other countries, with virotype C being overrepresented internationally. Correspondingly, an analysis of XbaI macrorestriction profiles revealed four major clusters, which were largely virotype specific. Certain epidemiological and clinical features corresponded with the virotype. Statistically significant virotype-specific associations included, for virotype B, older age and a lower frequency of infection (versus colonization), for virotype C, a higher frequency of infection, and for virotype D, younger age and community-acquired infections. In isolates of the O25b:H4-B2-ST131 clonal group, these findings uniquely define four main virotypes, which are internationally distributed, correspond with pulsed-field gel electrophoresis (PFGE) profiles, and exhibit distinctive clinical-epidemiological associationses
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEscherichia colies
dc.subjectInfecciones por escherichia colies
dc.subjectEpidemiología moleculares
dc.subjectPolimorfismo de longitud del fragmento de restricciónes
dc.subjectBeta-Lactamasases
dc.titleFour main virotypes among extended-spectrum-β-lactamase-producing isolates of Escherichia coli O25b:H4-B2-ST131: bacterial, epidemiological, and clinical characteristicses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.publisherversionhttp://jcm.asm.org/content/51/10/3358es
dc.identifier.doihttp://dx.doi.org/10.1128/JCM.01555-13es
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/38447

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