dc.creator | Rumbo, C. | |
dc.creator | Gato, E. | |
dc.creator | López, M. | |
dc.creator | Ruiz de Alegría, C. | |
dc.creator | Fernández-Cuenca, Felipe | |
dc.creator | Martínez-Martínez, Luis | |
dc.creator | Vila, Jordi | |
dc.creator | Pachón Díaz, Jerónimo | |
dc.creator | Cisneros, José Miguel | |
dc.creator | Rodríguez-Baño, Jesús | |
dc.creator | Pascual Hernández, Álvaro | |
dc.creator | Bou, Germán | |
dc.creator | Tomás, M. | |
dc.date.accessioned | 2016-02-18T18:21:35Z | |
dc.date.available | 2016-02-18T18:21:35Z | |
dc.date.issued | 2013-11 | |
dc.identifier.issn | 0066-4804 | es |
dc.identifier.uri | http://hdl.handle.net/11441/35789 | |
dc.description.abstract | Weinvestigated the mechanisms of resistance to carbapenems, aminoglycosides, glycylcyclines, tetracyclines, and quinolones in 90
multiresistant clinical strains of Acinetobacter baumannii isolated from two genetically unrelated A. baumannii clones: clone PFGEROC-
1 (53 strains producing the OXA-58B-lactamase enzyme and 18 strains with the OXA-24B-lactamase) and clone PFGE-HUI-1
(19 strains susceptible to carbapenems).Weused real-time reverse transcriptase PCR to correlate antimicrobial resistance (MICs) with
expression of genes encoding chromosomalB-lactamases (AmpC and OXA-51), porins (OmpA, CarO, Omp33, Dcap-like, OprB,
Omp25, OprC, OprD, and OmpW), and proteins integral to six efflux systems (AdeABC, AdeIJK, AdeFGH, CraA, AbeM, and AmvA).
Overexpression of the AdeABC system (level of expression relative to that by A. baumannii ATCC 17978, 30- to 45-fold) was significantly
associated with resistance to tigecycline, minocycline, and gentamicin and other biological functions. However, hyperexpression
of the AdeIJK efflux pump (level of expression relative to that by A. baumannii ATCC 17978, 8- to 10-fold) was significantly associated
only with resistance to tigecycline and minocycline (to which the TetB efflux system also contributed). TetB and TetA(39) efflux pumps
were detected in clinical strains and were associated with resistance to tetracyclines and doxycycline. The absence of the AdeABC system
and the lack of expression of other mechanisms suggest that tigecycline-resistant strains of the PFGE-HUI-1 clone may be associated
with a novel resistance-nodulation-cell efflux pump (decreased MICs in the presence of the inhibitor Phe-ArgB-naphthylamide
dihydrochloride) and the TetA(39) system. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Antimicrobial Agents and Chemotherapy 57(11) 5247–5257 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Beta-Lactamases | es |
dc.subject | Aminoglycosides | es |
dc.subject | Carbapenémicos | es |
dc.subject | Porinas | es |
dc.subject | Quinolonas | es |
dc.subject | Tetraciclinas | es |
dc.subject | Beta-Lactamasas | es |
dc.subject | Aminoglicósidos | es |
dc.title | Contribution of Efflux Pumps, Porins, and B-Lactamases to Multidrug Resistance in Clinical Isolates of Acinetobacter baumannii | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/AAC.00730-13 | |
dc.identifier.doi | 10.1128/AAC.00730-13 | |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/35789 | |