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dc.creatorRumbo, C.
dc.creatorGato, E.
dc.creatorLópez, M.
dc.creatorRuiz de Alegría, C.
dc.creatorFernández-Cuenca, Felipe
dc.creatorMartínez-Martínez, Luis
dc.creatorVila, Jordi
dc.creatorPachón Díaz, Jerónimo
dc.creatorCisneros, José Miguel
dc.creatorRodríguez-Baño, Jesús
dc.creatorPascual Hernández, Álvaro
dc.creatorBou, Germán
dc.creatorTomás, M.
dc.date.accessioned2016-02-18T18:21:35Z
dc.date.available2016-02-18T18:21:35Z
dc.date.issued2013-11
dc.identifier.issn0066-4804es
dc.identifier.urihttp://hdl.handle.net/11441/35789
dc.description.abstractWeinvestigated the mechanisms of resistance to carbapenems, aminoglycosides, glycylcyclines, tetracyclines, and quinolones in 90 multiresistant clinical strains of Acinetobacter baumannii isolated from two genetically unrelated A. baumannii clones: clone PFGEROC- 1 (53 strains producing the OXA-58B-lactamase enzyme and 18 strains with the OXA-24B-lactamase) and clone PFGE-HUI-1 (19 strains susceptible to carbapenems).Weused real-time reverse transcriptase PCR to correlate antimicrobial resistance (MICs) with expression of genes encoding chromosomalB-lactamases (AmpC and OXA-51), porins (OmpA, CarO, Omp33, Dcap-like, OprB, Omp25, OprC, OprD, and OmpW), and proteins integral to six efflux systems (AdeABC, AdeIJK, AdeFGH, CraA, AbeM, and AmvA). Overexpression of the AdeABC system (level of expression relative to that by A. baumannii ATCC 17978, 30- to 45-fold) was significantly associated with resistance to tigecycline, minocycline, and gentamicin and other biological functions. However, hyperexpression of the AdeIJK efflux pump (level of expression relative to that by A. baumannii ATCC 17978, 8- to 10-fold) was significantly associated only with resistance to tigecycline and minocycline (to which the TetB efflux system also contributed). TetB and TetA(39) efflux pumps were detected in clinical strains and were associated with resistance to tetracyclines and doxycycline. The absence of the AdeABC system and the lack of expression of other mechanisms suggest that tigecycline-resistant strains of the PFGE-HUI-1 clone may be associated with a novel resistance-nodulation-cell efflux pump (decreased MICs in the presence of the inhibitor Phe-ArgB-naphthylamide dihydrochloride) and the TetA(39) system.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofAntimicrobial Agents and Chemotherapy 57(11) 5247–5257es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBeta-Lactamaseses
dc.subjectAminoglycosideses
dc.subjectCarbapenémicoses
dc.subjectPorinases
dc.subjectQuinolonases
dc.subjectTetraciclinases
dc.subjectBeta-Lactamasases
dc.subjectAminoglicósidoses
dc.titleContribution of Efflux Pumps, Porins, and B-Lactamases to Multidrug Resistance in Clinical Isolates of Acinetobacter baumanniies
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.relation.publisherversionhttp://dx.doi.org/10.1128/AAC.00730-13
dc.identifier.doi10.1128/AAC.00730-13
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/35789

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional