dc.creator | Díaz, Miguel A. | |
dc.creator | Hernández-Bello, José R. | |
dc.creator | Rodríguez-Baño, Jesús | |
dc.creator | Martínez-Martínez, Luis | |
dc.creator | Calvo, Jorge | |
dc.creator | Blanco, Jorge | |
dc.creator | Pascual Hernández, Álvaro | |
dc.date.accessioned | 2016-02-18T16:07:04Z | |
dc.date.available | 2016-02-18T16:07:04Z | |
dc.date.issued | 2010-08 | |
dc.identifier.issn | 0095-1137 | es |
dc.identifier.uri | http://hdl.handle.net/11441/35183 | |
dc.description.abstract | The prevalence of extended-spectrum -lactamase (ESBL)-producing Escherichia coli (ESBLEC) in Spain
increased 8-fold from 2000 to 2006. ESBL type, clonal relationship, antimicrobial susceptibility, and clinical
data about infections caused by ESBLEC are evaluated in a second nationwide study developed in 2006. From
1008 clinical isolates obtained over 2 months from 44 hospitals, 254 were used for further analysis. ESBL
production was evaluated by synergy testing, PCR, and sequencing. Antimicrobial activity was evaluated by
microdilution. The clonal relationship was evaluated by repetitive extragenic palindromic-PCR (REP-PCR).
The O25b subtype and the new afa operon FM955459 were determined by triplex PCR in isolates producing
CTX-M-15. Multilocus sequence typing was performed on these isolates. A total of 72% of all ESBLs were of
the CTX-M type, 26.8% were of the SHV type, and 1.2% were of the TEM type. The most prevalent ESBLs were
CTX-M-14 (119 isolates), SHV-12 (68 isolates), CTX-M-15 (37 isolates), and CTX-M-9 (21 isolates). By
REP-PCR, 214 clones were detected. All but five CTX-M-15 ESBLEC isolates corresponded to the international
O25b/ST131 clone. This clone had not been detected in the first study (published in 2000). Epidemiological and
clinical features were studied in 304 representative patients. A total of 60% of the patients were older than 60
and had nonfatal underlying diseases, and 55% had recently received antibiotics. Urinary tract infections
accounted for 71% of cases, and 9% were bacteremic. There has been a significant increase in the prevalence
of ESBLEC in Spain, with most of these strains being CTX-M-producing isolates, including the pandemic
O25b-ST131. SHV-12-producing E. coli remains an important cause of community-acquired infection. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Clinical Microbiology 48 (8), 2840–2845 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | ESBL | es |
dc.subject | ESBLEC | es |
dc.subject | Escherichia coli | es |
dc.subject | Extended-spectrum-lactamase | es |
dc.subject | Antimicrobial | es |
dc.title | Diversity of Escherichia coli Strains Producing Extended-Spectrum B-Lactamases in Spain: Second Nationwide Study | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/jcm.02147-09 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JCM.02147-09 | |
dc.identifier.doi | 10.1128/JCM.02147-09 | |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/35183 | |