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Artículo
Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication
dc.creator | Cortés Ledesma, Felipe | |
dc.creator | Tous Rivera, Cristina | |
dc.creator | Aguilera López, Andrés | |
dc.date.accessioned | 2015-10-01T10:01:04Z | |
dc.date.available | 2015-10-01T10:01:04Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0305-1048 | es |
dc.identifier.issn | 1362-4962 | es |
dc.identifier.uri | http://hdl.handle.net/11441/29098 | |
dc.description.abstract | Homologous recombination (HR) is the major mechanism used to repair double-strand breaks (DSBs) that result from replication, but a study of repair of DSBs specifically induced during S-phase is lacking. Using an inverted-repeat assay in which a DSB is generated by the encountering of the replication fork with nicks, we can physically detect repair by sister-chromatid recombination (SCR) and intra-chromatid break-induced replication (IC-BIR). As expected, both events depend on Rad52, but, in contrast to previous data, both require Rad59, suggesting a prominent role of Rad59 in repair of replication-born DSBs. In the absence of Rad51, SCR is severely affected while IC-BIR increases, a phenotype that is also observed in the absence of Rad54 but not of its paralog Rdh54/Tid1. These data are consistent with SCR occurring by Rad51-dependent mechanisms assisted by Rad54, and indicate that in the absence of strand exchange-dependent SCR, breaks can be channeled to IC-BIR, which works efficiently in the absence of Rad51. Our study provides molecular evidence for inversions between repeats occurring by BIR followed by single-strand annealing (SSA) in the absence of strand exchange. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic acids research, 35(19), 6560-6570 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Molecular Biology | es |
dc.title | Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/gkm488 | es |
dc.identifier.doi | http://dx.doi.org/10.1093/nar/gkm488 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/29098 |
Ficheros | Tamaño | Formato | Ver | Descripción |
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6560.full.pdf | 5.737Mb | [PDF] | Ver/ | |