Artículo
Persulfidation of ATG18a regulates autophagy underER stress in Arabidopsis
Autor/es | Aroca Aguilar, Ángeles
Yruela Guerrero, Inmaculada Gotor Martínez, Cecilia Bassham, Diane C. |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2021-05 |
Fecha de depósito | 2024-07-19 |
Publicado en |
|
Resumen | Hydrogen sulfide (H2S) is an endogenously generated gaseous signaling molecule, which recently has been implicated in autophagy regulation in both plants and mammals through persulfidation of specific targets. Persulfidation ... Hydrogen sulfide (H2S) is an endogenously generated gaseous signaling molecule, which recently has been implicated in autophagy regulation in both plants and mammals through persulfidation of specific targets. Persulfidation has been suggested as the molecular mechanism through which sulfide regulates autophagy in plant cells. ATG18a is a core autophagy component that is required for bulk autophagy and also for reticulophagy during endoplasmic reticulum (ER) stress. In this research, we revealed the role of sulfide in plant ER stress responses as a negative regulator of autophagy. We demonstrate that sulfide regulates ATG18a phospholipid-binding activity by reversible persulfidation at Cys103, and that this modification activates ATG18a binding capacity to specific phospholipids in a reversible manner. Our findings strongly suggest that persulfidation of ATG18a at C103 regulates autophagy under ER stress, and that the impairment of persulfidation affects both the number and size of autophagosomes. |
Agencias financiadoras | European Union (UE). H2020 European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Agencia Estatal de Investigación. España National Institutes of Health. United States |
Identificador del proyecto | 834120
US-125578 PID2019-109785GB-I00 R01GM120316-01A1 |
Cita | Aroca Aguilar, Á., Yruela Guerrero, I., Gotor Martínez, C. y Bassham, D.C. (2021). Persulfidation of ATG18a regulates autophagy underER stress in Arabidopsis. Proceedings of the National Academy of Sciences (PNAS), 118 (20), e2023604118. https://doi.org/10.1073/pnas.2023604118. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
10.1073pnas.2023604118.pdf | 3.932Mb | [PDF] | Ver/ | Versión aceptada |