Article
An Actin Remodeling Role for Arabidopsis Processing Bodies Revealed by their Proximity Interactome
Author/s | Liu, Chen
Mentzelopoulou, Andriani Muhammad, Amna Volkov, Andriy Weijers, Dolf Gutiérrez Beltrán, Emilio Moschou, Panagiotis N. |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2023 |
Deposit Date | 2024-07-03 |
Published in |
|
Abstract | Cellular condensates can comprise membrane-less ribonucleoprotein assemblies with liquid-like properties. These cellular condensates influence various biological outcomes, but their liquidity hampers their isolation and ... Cellular condensates can comprise membrane-less ribonucleoprotein assemblies with liquid-like properties. These cellular condensates influence various biological outcomes, but their liquidity hampers their isolation and characterization. Here, we investigated the composition of the condensates known as processing bodies (PBs) in the model plant Arabidopsis thaliana through a proximity-biotinylation proteomics approach. Using in situ protein–protein interaction approaches, genetics and high-resolution dynamic imaging, we show that processing bodies comprise networks that interface with membranes. Surprisingly, the conserved component of PBs, DECAPPING PROTEIN 1 (DCP1), can localize to unique plasma membrane subdomains including cell edges and vertices. We characterized these plasma membrane interfaces and discovered a developmental module that can control cell shape. This module is regulated by DCP1, independently from its role in decapping, and the actin-nucleating SCAR–WAVE complex, whereby the DCP1–SCAR–WAVE interaction confines and enhances actin nucleation. This study reveals an unexpected function for a conserved condensate at unique membrane interfaces. |
Funding agencies | Carl Tryggers Foundation European Union (UE) Vetenskapsradet (VR) research council FORMAS research council Helge Ax:son Johnsons stiftelse Hellenic Foundation of Research and Innovation (HFRI) European Research Council (ERC) Ministerio de Ciencia e Innovación (MICIN). España |
Project ID. | 15
17:336 872969 21679000 22924-000 F19-0419 06526 833867 PID2020-119737GA-I00 |
Citation | Liu, C., Mentzelopoulou, A., Muhammad, A., Volkov, A., Weijers, D., Gutiérrez Beltrán, E. y Moschou, P.N. (2023). An Actin Remodeling Role for Arabidopsis Processing Bodies Revealed by their Proximity Interactome. EMBO Journal, 42 (9), e111885. https://doi.org/https://dx.doi.org/10.15252/embj.2022111885. |
Files | Size | Format | View | Description |
---|---|---|---|---|
An actin remodeling.pdf | 5.003Mb | [PDF] | View/ | |