dc.creator | Pulido, Marina R. | es |
dc.creator | García Montaner, Andrea | es |
dc.creator | López Cerero, Lorena | es |
dc.creator | Fernández Cuenca, Felipe Manuel | es |
dc.creator | Gutiérrez-Fernández, José | es |
dc.creator | Pascual Hernández, Álvaro | es |
dc.date.accessioned | 2024-06-26T08:40:30Z | |
dc.date.available | 2024-06-26T08:40:30Z | |
dc.date.issued | 2022-07 | |
dc.identifier.issn | 0021-9193 | es |
dc.identifier.issn | 1098-5530 | es |
dc.identifier.uri | https://hdl.handle.net/11441/160873 | |
dc.description.abstract | This study characterizes a new genetic structure containing a multicopy of a bla(VIM-2) variant with an A676C substitution, bla(VIM-63). This gene was detected on the chromosome of two carbapenem-resistant clinical strains of Citrobacter freundii ST22 recovered from two patients, separated by a 6-month period, and previously in Pseudomonas aeruginosa ST2242 from the same hospital unit. Short-read sequencing was used to characterize the new variant in both species, and long-read sequencing was used to characterize the genome of C. freundii. On the P. aeruginosa chromosome, the bla(VIM-63) gene was inserted between ISPsy 42-type sequences, flanked by an intl1 sequence, nearby aph(3′)-VI, and sul1. On the C. freundii chromosome, the bla(VIM-63) gene was inserted into a Tn6230-like transposon as a stable five-tandem-repeat multimer, flanked by the same intl1 as in P. aeruginosa. This structure was stable across subcultures and did not change in the presence of carbapenems. The bla(VIM-63) gene was cloned into the pCR-Blunt plasmid to study antimicrobial susceptibility patterns and into pET29a for kinetic activity analysis. VIM-63 showed higher K(m) values than VIM-2 for ceftazidime and cefepime and higher k(cat) values for cefotaxime, ceftazidime, imipenem, and ertapenem, without differences in MIC values. This is the first study to describe this new variant, VIM-63, in two different species with a chromosomal location integrated into different mobile elements and the first to describe a stable multimer of a metallo-β-lactamase. Despite the amino acid substitution, the susceptibility pattern of the new variant was similar to that of VIM-2. IMPORTANCE: VIM group metallo-β-lactamases are usually captured by IntI1 integrases. This work describes the detection for the first time of a novel, previously unknown variant of VIM-2, VIM-63. This carbapenemase has been found on the chromosome of two different species, Citrobacter freundii and Pseudomonas aeruginosa, from the same hospital. The adjacent genetic environment of the bla(VIM-63) gene would indicate that the capture of this gene by IntI1 has occurred in two different genetic events in each of the species, and in one there has been a stable integration of tandem copies of this gene. | es |
dc.description.sponsorship | European Development Regional Fund "A way to achieve Europe," Operative program Intelligent Growth 2014 to 2020 | es |
dc.description.sponsorship | nstituto de Salud Carlos III, Subdireccion General de Redes y Centros de Investigacion Cooperativa, Ministerio de Economia, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI) RD16/0016/0001 | es |
dc.description.sponsorship | Plan Nacional de I+D+i 2013-016 | es |
dc.description.sponsorship | VIPPI-US fellowship from the University of Seville | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | VIM-63 | es |
dc.subject | VIM-2 variant | es |
dc.subject | Carbapenemase | es |
dc.subject | Multimer | es |
dc.subject | WGS | es |
dc.title | Identification of a Stable Chromosomal Tandem Multicopy of bla(VIM-63), a New bla(VIM-2) Carbapenemase | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | https://journals.asm.org/doi/epub/10.1128/jb.00088-22 | es |
dc.identifier.doi | 10.1128/jb.00088-22 | es |
dc.contributor.group | Universidad de Sevilla. CTS210: Resistencia a Antimicrobianos | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 204 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 9 | es |