Article
Doped electrospinned material-guides high efficiency regional bone regeneration
Author/s | Toledano, Manuel
Vallecillo, Cristina Serrera Figallo, María de los Ángeles Vallecillo-Rivas, Marta Gutiérrez Corrales, Aída Lynch, Christopher D. Toledano-Osorio, Manuel |
Department | Universidad de Sevilla. Departamento de Estomatología |
Publication Date | 2023-03-30 |
Deposit Date | 2024-06-19 |
Published in |
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Abstract | The main target of bone tissue engineering is to design biomaterials that support bone
regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-
co-(HEA)2 loaded with 5% wt of SiO2
-nanoparticles ... The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3- co-(HEA)2 loaded with 5% wt of SiO2 -nanoparticles (Si-M) were doped with zinc (Zn-Si-M) or doxycycline (Dox-Si-M). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and then they were covered with the membranes. After six weeks, a histological analysis (toluidine blue technique) was employed to determine bone cell population as osteoblasts, osteoclasts, osteocytes, M1 and M2 macrophages and vasculature. Membranes covering the bone defect determined a higher count of bone cells and blood vessels than in the sham group at the top regions of the defect. Pro-inflammatory M1 appeared in a higher number in the top regions than in the bottom regions, when Si-M and Dox-Si-M were used. Samples treated with Dox-Si-M showed a higher amount of anti-inflammatory and pro-regenerative M2 macrophages. The M1/M2 ratio obtained its lowest value in the absence of membranes. On the top regions, osteoblasts were more abundant when using Si-M and Zn-Si-M. Osteoclasts were equally distributed at the central and lateral regions. The sham group and samples treated with Zn-Si-M attained a higher number of osteocytes at the top regions. A preferential osteoconductive, osteoinductive and angiogenic clinical environment was created in the vicinity of the membrane placed on critical bone defects. |
Citation | Toledano, M., Vallecillo, C., Serrera Figallo, M.d.l.Á., Vallecillo-Rivas, M., Gutiérrez Corrales, A., Lynch, C.D. y Toledano-Osorio, M. (2023). Doped electrospinned material-guides high efficiency regional bone regeneration. Plymers, 15 (7), 1726. https://doi.org/10.3390/polym15071726. |
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