dc.creator | Rubio Contreras, Diana | es |
dc.creator | Gómez Herreros, Fernando | es |
dc.date.accessioned | 2024-05-21T11:07:01Z | |
dc.date.available | 2024-05-21T11:07:01Z | |
dc.date.issued | 2023-11-09 | |
dc.identifier.citation | Rubio Contreras, D. y Gómez Herreros, F. (2023). TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription. Nature Communications, 14 (1), 6940. https://doi.org/10.1038/s41467-023-42622-7. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | https://hdl.handle.net/11441/158734 | |
dc.description.abstract | DNA topoisomerase I (TOP1) removes torsional stress by transiently cutting one DNA strand. Such cuts are rejoined by TOP1 but can occasionally become abortive generating permanent protein-linked single strand breaks (SSBs). The repair of these breaks is initiated by tyrosyl-DNA phosphodiesterase 1 (TDP1), a conserved enzyme that unlinks the TOP1 peptide from the DNA break. Additionally, some of these SSBs can result in double strand breaks (DSBs) either during replication or by a poorly understood transcription-associated process. In this study, we identify these DSBs as a source of genome rearrangements, which are suppressed by TDP1. Intriguingly, we also provide a mechanistic explanation for the formation of chromosomal translocations unveiling an error-prone pathway that relies on the MRN complex and canonical non-homologous end-joining. Collectively, these data highlight the threat posed by TOP1-induced DSBs during transcription and demonstrate the importance of TDP1-dependent end-joining in protecting both gene transcription and genome stability. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación (MCIN). España, Agencia Estatal de Investigación (AEI) PID2019-105212GB-I00 | es |
dc.description.sponsorship | Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía, España P20_00561 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Nature Research | es |
dc.relation.ispartof | Nature Communications, 14 (1), 6940. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | PID2019-105212GB-I00 | es |
dc.relation.projectID | P20_00561 | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-023-42622-7 | es |
dc.identifier.doi | 10.1038/s41467-023-42622-7 | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 14 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 6940 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.contributor.funder | Junta de Andalucía | es |