dc.creator | Galvez-Benitez, Lydia | es |
dc.creator | Ortiz de la Rosa, Jose Manuel | es |
dc.creator | Rodríguez-Villodres, Angel | es |
dc.creator | Casimiro-Soriguer, Carlos S. | es |
dc.creator | Molina-Panadero, Irene | es |
dc.creator | Alvarez-Marín, Rocío | es |
dc.creator | Bonnin, Remy A | es |
dc.creator | Naas, Thierry | es |
dc.creator | Pachón Díaz, Jerónimo | es |
dc.creator | Cisneros, José Miguel | es |
dc.creator | Lepe Jiménez, José Antonio | es |
dc.creator | Smani, Younes | es |
dc.date.accessioned | 2024-05-20T10:47:25Z | |
dc.date.available | 2024-05-20T10:47:25Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0163-4453 | es |
dc.identifier.issn | 1532-2742 | es |
dc.identifier.uri | https://hdl.handle.net/11441/158616 | |
dc.description.abstract | Piperacillin-tazobactam resistance (P/T-R) is increasingly reported among Escherichia coli isolates. Although in vitro experiments have suggested that blaTEM gene plays a key role in the P/T-R acquisition, no clinical in vivo study has yet confirmed the role of blaTEM or other genes. Therefore, we aimed to identify the mechanisms underlying P/T-R by following up patients with E. coli complicated intra-abdominal infections (cIAI) who experienced P/T treatment failure. Four pairs of strains, clonally related from four patients, were isolated both before and after treatment with P/T dosed at 4 g/0.5 g intravenously. The P/T MIC was tested using broth microdilution, and β-lactamase activity was determined in these isolates. Whole-genome sequencing (WGS) was performed to decipher the role of blaTEM and other genes associated with P/T-R. Changes in the outer membrane protein (OMP) profile were analyzed using SDS-PAGE, and blaTEM and ompC transcription levels were measured by RT-qPCR. In addition, in vitro competition fitness was performed between each pairs of strains (P/T-susceptible vs. P/T-resistant). We found a higher copy number of blaTEM gene in P/T-R isolates, generated by three different genetic events: (1) IS26-mediated duplication of the blaTEM gene, (2) generation of a small multicopy plasmid (ColE-like) carrying blaTEM, and (3) adaptive evolution via reduction of plasmid size, leading to a higher plasmid copy number. Moreover, two P/T-R strains showed reduced expression of OmpC. This study describes the mechanisms involved in the acquisition of P/T-R by E. coli in patients with cIAI. The understanding of P/T-R evolution is crucial for effectively treating infected patients and preventing the spread of resistant microorganisms. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Complicated intra-abdominal infection | es |
dc.subject | Escherichia coli | es |
dc.subject | OmpC | es |
dc.subject | Piperacillin-tazobactam | es |
dc.subject | Resistance | es |
dc.subject | ß-lactamase | es |
dc.title | Role of blaTEM and OmpC in the piperacillin-tazobactam resistance evolution by E. coli in patients with complicated intra-abdominal infection | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | CPII20/00018 | es |
dc.relation.publisherversion | https://www.journalofinfection.com/article/S0163-4453(23)00377-8/fulltext | es |
dc.identifier.doi | 10.1016/j.jinf.2023.07.005 | es |
dc.journaltitle | Journal of Infection | es |
dc.publication.volumen | 87 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | 220 | es |
dc.publication.endPage | 229 | es |
dc.contributor.funder | Economa y Competitividad, Spain | es |
dc.contributor.funder | Universidad Pablo de Olavide | es |
dc.description.awardwinning | Premio Mensual Publicación Científica Destacada de la US. Facultad de Medicina | |
dc.description.awardwinning | Premio Anual Publicación Científica Destacada de la US. Facultad de Medicina | |