dc.creator | Zhao, Yichao | es |
dc.creator | Tabet, Daniel | es |
dc.creator | Rubio Contreras, Diana | es |
dc.creator | Lao, Linjiang | es |
dc.creator | Kousholt, Arne Nedergaard | es |
dc.creator | Weile, Jochen | es |
dc.creator | Gómez Herreros, Fernando | es |
dc.creator | Durocher, Daniel | es |
dc.date.accessioned | 2024-05-17T10:27:50Z | |
dc.date.available | 2024-05-17T10:27:50Z | |
dc.date.issued | 2023-08-03 | |
dc.identifier.citation | Zhao, Y., Tabet, D., Rubio Contreras, D., Lao, L., Kousholt, A.N., Weile, J.,...,Durocher, D. (2023). Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens. Molecular Cell, 83 (15), 2792-2809. https://doi.org/10.1016/j.molcel.2023.06.025. | |
dc.identifier.issn | 1097-2765 | es |
dc.identifier.issn | 1097-4164 | es |
dc.identifier.uri | https://hdl.handle.net/11441/158498 | |
dc.description.abstract | To maintain genome integrity, cells must accurately duplicate their genome and repair DNA lesions when they
occur. To uncover genes that suppress DNA damage in human cells, we undertook flow-cytometry-based
CRISPR-Cas9 screens that monitored DNA damage. We identified 160 genes whose mutation caused spontaneous DNA damage, a list enriched in essential genes, highlighting the importance of genomic integrity for
cellular fitness. We also identified 227 genes whose mutation caused DNA damage in replication-perturbed
cells. Among the genes characterized, we discovered that deoxyribose-phosphate aldolase DERA suppresses DNA damage caused by cytarabine (Ara-C) and that GNB1L, a gene implicated in 22q11.2 syndrome,
promotes biogenesis of ATR and related phosphatidylinositol 3-kinase-related kinases (PIKKs). These results implicate defective PIKK biogenesis as a cause of some phenotypes associated with 22q11.2 syndrome. The phenotypic mapping of genes that suppress DNA damage therefore provides a rich resource
to probe the cellular pathways that influence genome maintenance | es |
dc.description.sponsorship | National Institutes of Health (NIH). Estados Unidos RM1HG010461 - UM1HG011989 | es |
dc.description.sponsorship | Canadian Cancer Society 705644 | es |
dc.description.sponsorship | Canadian Institutes of Health Research CIHR PJT-180438 - FDN159926 | es |
dc.format | application/pdf | es |
dc.format.extent | 17 | es |
dc.language.iso | eng | es |
dc.publisher | Cell Press | es |
dc.relation.ispartof | Molecular Cell, 83 (15), 2792-2809. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | RM1HG010461 | es |
dc.relation.projectID | UM1HG011989 | es |
dc.relation.projectID | 705644 | es |
dc.relation.projectID | PJT-180438 | es |
dc.relation.projectID | FDN159926 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.molcel.2023.06.025 | es |
dc.identifier.doi | 10.1016/j.molcel.2023.06.025 | es |
dc.journaltitle | Molecular Cell | es |
dc.publication.volumen | 83 | es |
dc.publication.issue | 15 | es |
dc.publication.initialPage | 2792 | es |
dc.publication.endPage | 2809 | es |
dc.contributor.funder | National Institutes of Health (NIH). Estados Unidos | es |
dc.contributor.funder | Canadian Cancer Society. Canada | es |
dc.contributor.funder | Canadian Institutes of Health Research CIHR | es |