Artículo
Unlocking the Potential of Iron-Containing Mesoporous Bioactive Glasses: Orchestrating Osteogenic Differentiation in Bone Marrow Mesenchymal Stem Cells and Osteoblasts
Autor/es | Zhou, Tian
Xu, Zeqian Sun, Haishui Beltrán, Ana M. Nawaz, Qaisar Sui, Baiyan Boccaccini, Aldo R. Zheng, Kai |
Departamento | Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte |
Fecha de publicación | 2024-05 |
Fecha de depósito | 2024-05-10 |
Resumen | Iron (Fe) is a crucial element in the human body, playing a significant role in bone metabolism.
The release of Fe ions at bone defect sites can promote bone regeneration. In this study, we
synthesized Fe-containing ... Iron (Fe) is a crucial element in the human body, playing a significant role in bone metabolism. The release of Fe ions at bone defect sites can promote bone regeneration. In this study, we synthesized Fe-containing mesoporous bioactive glasses (Fe-MBGs) in SiO₂-CaO-Fe₂O₃ composition using a sol-gel method. Regardless of the amount of incorporated Fe₂O₃ (up to 5 mol%), the Fe-MBGs maintained a mesoporous structure, and the inclusion of Fe₂O₃ did not alter their amorphous characteristics. However, the presence of Fe₂O₃ led to a reduction in both pore volume and specific surface area of the Fe-MBGs. Notably, Fe-MBGs demonstrated degradability in physiological fluids and could sustain release of Si, Fe, and Ca ions. Higher concentrations of incorporated Fe₂O₃ were found to reduce the degradation of Fe-MBGs. All Fe-MBGs exhibited favorable bioactivity, as evidenced by the rapid formation of hydroxyapatite when exposed to simulated body fluid. Fe-MBGs also demonstrated concentration-dependent effects on BMSCs and Saos-2 cells. Extracts of Fe-MBGs at 0.1 and 1 mg/mL exhibited non-cytotoxicity and promoted cell proliferation. Additionally, extracts of Fe-MBGs at 1 mg/mL significantly enhanced the alkaline phosphatase activity of BMSCs and Saos-2 cells, along with an upregulation of the expression of osteogenesis-related genes. These findings unlock the significant potential of Fe-MBGs as functional biomaterials for bone regeneration applications. The controlled release of Fe ions from these mesoporous bioactive glasses orchestrates osteogenic differentiation in bone marrow mesenchymal stem cells and osteoblasts. |
Agencias financiadoras | Universidad de Sevilla |
Identificador del proyecto | VIIPPI |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
CS_beltran-custodio_2024_Unloc ... | 634.9Kb | [PDF] | Este documento no está disponible a texto completo hasta el 2026-06-01 . Para más información póngase en contacto con idus@us.es. | Postprint. Accepted Version |