Article
Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence
Author/s | Ruiz Solaní, Nerea
Salguero Linares, José Armengot, Laia Santos, Jaime Pallarèes, Irantzu van Midden, Katarina P. Phukkan, Ujjal J. Koyuncu, Seda Bòrras-Bisa, Julia Gutiérrez Beltrán, Emilio Coll, Nuria S. |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2023-07-01 |
Deposit Date | 2024-02-22 |
Published in |
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Abstract | Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute to maintaining protein homeostasis. These membraneless organelles are dynamic, disassembling once the ... Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute to maintaining protein homeostasis. These membraneless organelles are dynamic, disassembling once the stress is no longer present. Persistence of SGs due to mutations or chronic stress has been often related to age-dependent protein-misfolding diseases in animals. Here, we find that the metacaspase MC1 is dynamically recruited into SGs upon proteotoxic stress in Arabidopsis (Arabidopsis thaliana). Two predicted disordered regions, the prodomain and the 360 loop, mediate MC1 recruitment to and release from SGs. Importantly, we show that MC1 has the capacity to clear toxic protein aggregates in vivo and in vitro, acting as a disaggregase. Finally, we demonstrate that overexpressing MC1 delays senescence and this phenotype is dependent on the presence of the 360 loop and an intact catalytic domain. Together, our data indicate that MC1 regulates senescence through its recruitment into SGs and this function could potentially be linked to its remarkable protein aggregate-clearing activity. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España Deutsche Forschungsgemeinschaft (DFG) Else Kröner-Fresenius-Stiftung |
Project ID. | PID2019-108595RB-I00
AGL2016-78002-R BES-2017-080210 SEV-2015-0533 CEX2019-000902-S PID2020-119737GA-I00 PID2019-105017RB-I00 CEDAC EXC 2030-390661388 CEPAS EXC-2048/ 1–390686111 2021-EKSE.95 |
Citation | Ruiz Solaní, N., Salguero Linares, J., Armengot, L., Santos, J., Pallarèes, I., van Midden, K.P.,...,Coll, N.S. (2023). Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates, and delays senescence. Plant Cell, 35 (9), 3325-3344. https://doi.org/10.1093/plcell/koad172. |
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