Article
Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus
Author/s | Trush, Kristina
Gavurová, Martina Monje Rueda, María Dolores Kolarčik, Vladislav Bačovčinová, Michaela Betti, Marco Paľove-Balang, Peter |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2024-03 |
Deposit Date | 2024-02-20 |
Published in |
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Abstract | Nitrogen availability is key factor for plant metabolism and has a strong impact on the biosynthesis of secondary plant metabolites. A number of these metabolites are involved in the response to stress. In this paper, we ... Nitrogen availability is key factor for plant metabolism and has a strong impact on the biosynthesis of secondary plant metabolites. A number of these metabolites are involved in the response to stress. In this paper, we investigated the effect of different N conditions in leaves of Lotus corniculatus. Low nitrogen conditions strongly enhanced the biosynthesis of phenolic compounds in leaves, inducing the expression genes encoding for key enzymes of phenolic metabolism such as phenylalanine ammonia lyase, chalcone synthase and isoflavone synthase. Kaempferol and quercetin glycosides as well as isoflavanes accumulated in leaves. Surprisingly, if the nitrogen availability was limited, but without nitrogen deprivation symptoms, an increase in the levels of phenylpropane acids and a decrease in the levels of kaempferol glycosides was detected. Especially the decrease in malonylated kaempferol derivatives and the kaempferol glycosides substituted by rhamnose in the 3-O position. The L. corniculatus malonyltransferase 1 gene is likely involved in the malonylation of the kaempferol glycosides. |
Funding agencies | Ministry of Education, Science, Research and Sport. Slovak Republic Ministerio de Ciencia e Innovación (MICIN). España Ministerio de Ciencia, Innovación y Universidades (MICINN). España |
Project ID. | VEGA 1/ 0291/20
PID2021-122353OB-I00 RTI-2018-093571-B100 |
Citation | Trush, K., Gavurová, M., Monje Rueda, M.D., Kolarčik, V., Bačovčinová, M., Betti, M. y Paľove-Balang, P. (2024). Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus. Plant Stress, 11, 100336. https://doi.org/10.1016/j.stress.2023.100336. |
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