Artículo
Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment
Autor/es | McConnell, Michael J.
Pérez Ordóñez, Ana Pérez-Romero, Pilar Valencia, R. Lepe Jiménez, José Antonio Vázquez-Barba, Isabel Pachón Díaz, Jerónimo |
Departamento | Universidad de Sevilla. Departamento de Medicina Preventiva y Salud Pública Universidad de Sevilla. Departamento de Medicina Universidad de Sevilla. Departamento de Microbiología |
Fecha de publicación | 2012 |
Fecha de depósito | 2024-01-24 |
Publicado en |
|
Resumen | A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR ... A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria. |
Cita | McConnell, M.J., Pérez Ordóñez, A., Pérez-Romero, P., Valencia, R., Lepe Jiménez, J.A., Vázquez-Barba, I. y Pachón Díaz, J. (2012). Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment. Journal of Clinical Microbiology, 50 (4), 1412-1414. https://doi.org/10.1128/jcm.06566-11. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Quantitative Real-Time .pdf | 350.3Kb | [PDF] | Ver/ | |