Article
Time-Dependent Collagen Fibered Structure in the Early Distraction Callus: Imaging Characterization and Mathematical Modeling
Author/s | Blázquez Carmona, Pablo
Sanz Herrera, José Antonio Mora Macías, Juan Morgaz, Juan Domínguez Abascal, Jaime Reina Romo, Esther |
Department | Universidad de Sevilla. Departamento de Mecánica de Medios Continuos y Teoría de Estructuras |
Publication Date | 2022-12 |
Deposit Date | 2024-01-22 |
Published in |
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Abstract | Collagen is a ubiquitous protein present in regenerating bone tissues that experiences multiple biological phenomena during distraction osteogenesis until the deposition of phosphate crystals. This work combines fluorescence ... Collagen is a ubiquitous protein present in regenerating bone tissues that experiences multiple biological phenomena during distraction osteogenesis until the deposition of phosphate crystals. This work combines fluorescence techniques and mathematical modeling to shed light on the mechano-structural processes behind the maturation and accommodation-to-mineralization of the callus tissue. Ovine metatarsal bone calluses were analyzed through confocal images at different stages of the early distraction osteogenesis process, quantifying the fiber orientation distribution and mean intensity as fiber density measure. Likewise, a mathematical model based on the experimental data was defined to micromechanically characterize the apparent stiffening of the tissue within the distracted callus. A reorganization of the fibers around the distraction axis and increased fiber density were found as the bone fragments were gradually separated. Given the degree of significance between the mathematical model and previous in vivo data, reorganization, densification, and bundle maturation phenomena seem to explain the apparent mechanical maturation observed in the tissue theoretically. © 2022, The Author(s). |
Funding agencies | Ministerio de Economia, Industria y Competitividad (MINECO). España Agencia Estatal de Investigación. España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
Project ID. | DPI2017-82501-P
PID2020-113790RB-I00/AEI/ 10.13039/501100011033 US-1261691 |
Citation | Blázquez-Carmona, P., Sanz-Herrera, J.A., Mora-Macías, J., Morgaz, J., Domínguez, J. y Reina-Romo, E. (2022). Time-Dependent Collagen Fibered Structure in the Early Distraction Callus: Imaging Characterization and Mathematical Modeling. Annals of Biomedical Engineering, 50 (12), 1768-1809. https://doi.org/10.1007/s10439-022-02992-3. |
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