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Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure
dc.creator | Kazakova, Julia | es |
dc.creator | Villar Navarro, Mercedes | es |
dc.creator | Pérez Bernal, Juan Luis | es |
dc.creator | Ramos Payán, María Dolores | es |
dc.creator | Bello López, Miguel Ángel | es |
dc.creator | Fernández Torres, Rut | es |
dc.date.accessioned | 2024-01-12T13:43:24Z | |
dc.date.available | 2024-01-12T13:43:24Z | |
dc.date.issued | 2021-07 | |
dc.identifier.citation | Kazakova, J., Villar Navarro, M., Pérez Bernal, J.L., Ramos Payán, M.D., Bello López, M.Á. y Fernández Torres, R. (2021). Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure. Microchemical journal, 166, 106193. https://doi.org/10.1016/j.microc.2021.106193. | |
dc.identifier.issn | 0026-265X | es |
dc.identifier.issn | 1095-9149 | es |
dc.identifier.uri | https://hdl.handle.net/11441/153302 | |
dc.description.abstract | In this work, the determination of 2,4-, 2,5- and 2,6-dinitrophenols and the identification of some of their metabolites in human urine and saliva is proposed. A three phase hollow fiber based liquid phase microextraction prior to ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry allowed low detection and quantitation limits of the target analytes, as well as the investigation and tentatively identification of some metabolites by accurate mass full-spectrum measurements. The chromatographic separation was accomplished on an Acquity BEH C18 column (50 mm × 2.1 mm i.d., 1.7 μm particle size) at 25 ºC using water and acetonitrile (with 0.1 % (v/v) formic acid) 20:80 v/v as mobile phase, at a flow rate of 0.5 mL/min in isocratic elution mode for 5 min. Hollow fiber liquid phase microextraction was achieved at donor phase pH 2, acceptor phase pH 13 and dihexylether as supported liquid membrane. Under the optimal conditions, detection limits for 2,4-, 2,5- and 2,6-dinitrophenol, respectively, were 0.18 μg·L-1, 0.38 μg·L-1 and 0.14 μg·L-1 in urine samples and 0.32 μg·L-1, 0.67 μg·L-1 and 0.24 μg·L-1 in saliva samples. The proposed methodology was applied on urine and saliva samples from laboratory staff likely to be or not occupationally exposed to dinitrophenols, finding quantitative levels of 2,4- and 2,6-dinitrophenol and identifying some metabolites previously reported in literature. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad (MINECO). España CTM2015-67902-C-1-P | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación (MICIN). España GC2018-096608-B-C22 | es |
dc.description.sponsorship | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) GC2018-096608-B-C22 | es |
dc.format | application/pdf | es |
dc.format.extent | 23 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier Inc. | es |
dc.relation.ispartof | Microchemical journal, 166, 106193. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hollow fiber liquid phase microextraction | es |
dc.subject | HF-LPME | es |
dc.subject | Liquid chromatography quadrupole time-of-flight | es |
dc.subject | Dinitrophenols | es |
dc.subject | Human urine | es |
dc.subject | Human saliva | es |
dc.title | Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Analítica | es |
dc.relation.projectID | CTM2015-67902-C-1-P | es |
dc.relation.projectID | GC2018-096608-B-C22 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.microc.2021.106193 | es |
dc.identifier.doi | 10.1016/j.microc.2021.106193 | es |
dc.journaltitle | Microchemical journal | es |
dc.publication.volumen | 166 | es |
dc.publication.initialPage | 106193 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |