Artículo
Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure
Autor/es | Kazakova, Julia
Villar Navarro, Mercedes Pérez Bernal, Juan Luis Ramos Payán, María Dolores Bello López, Miguel Ángel Fernández Torres, Rut |
Departamento | Universidad de Sevilla. Departamento de Química Analítica |
Fecha de publicación | 2021-07 |
Fecha de depósito | 2024-01-12 |
Publicado en |
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Resumen | In this work, the determination of 2,4-, 2,5- and 2,6-dinitrophenols and the identification of some of their metabolites in human urine and saliva is proposed. A three phase hollow fiber based liquid phase microextraction ... In this work, the determination of 2,4-, 2,5- and 2,6-dinitrophenols and the identification of some of their metabolites in human urine and saliva is proposed. A three phase hollow fiber based liquid phase microextraction prior to ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry allowed low detection and quantitation limits of the target analytes, as well as the investigation and tentatively identification of some metabolites by accurate mass full-spectrum measurements. The chromatographic separation was accomplished on an Acquity BEH C18 column (50 mm × 2.1 mm i.d., 1.7 μm particle size) at 25 ºC using water and acetonitrile (with 0.1 % (v/v) formic acid) 20:80 v/v as mobile phase, at a flow rate of 0.5 mL/min in isocratic elution mode for 5 min. Hollow fiber liquid phase microextraction was achieved at donor phase pH 2, acceptor phase pH 13 and dihexylether as supported liquid membrane. Under the optimal conditions, detection limits for 2,4-, 2,5- and 2,6-dinitrophenol, respectively, were 0.18 μg·L-1, 0.38 μg·L-1 and 0.14 μg·L-1 in urine samples and 0.32 μg·L-1, 0.67 μg·L-1 and 0.24 μg·L-1 in saliva samples. The proposed methodology was applied on urine and saliva samples from laboratory staff likely to be or not occupationally exposed to dinitrophenols, finding quantitative levels of 2,4- and 2,6-dinitrophenol and identifying some metabolites previously reported in literature. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Ministerio de Ciencia e Innovación (MICIN). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
Identificador del proyecto | CTM2015-67902-C-1-P
GC2018-096608-B-C22 |
Cita | Kazakova, J., Villar Navarro, M., Pérez Bernal, J.L., Ramos Payán, M.D., Bello López, M.Á. y Fernández Torres, R. (2021). Urine and saliva biomonitoring by HF-LPME-LC/MS to assess dinitrophenols exposure. Microchemical journal, 166, 106193. https://doi.org/10.1016/j.microc.2021.106193. |